Spatial heterogeneity of myocardial blood flow predicts necrosis both prior to coronary occlusion and after reperfusion in conscious pigs

C. H. Huang, Y. T. Shen, S. J. Kim, B. Ghaleh, R. Kudej, Stephen Vatner

Research output: Contribution to journalArticle

Abstract

Myocardial blood flow may average 1.0 ml/min/g, but actually ranges from approximately 0.2-2.0 ml/min/g in different myocardial samples due to spatial heterogeneity. We recently observed that the spatial heterogeneity could predict necrosis in baboons with coronary artery occlusion (CAO) and reperfusion (CAR). The goal of this study was to determine if necrosis could be predicted using spatial heterogeneity analyses either prior to CAO or early after CAR in conscious pigs. Accordingly, the effects of CAO for 90 minutes followed by 4-7 days of CAR were examined in 6 conscious pigs instrumented with aortic, left atrial catheters and CA occluders. Myocardial blood flow was measured by radioactive microspheres before CAO, during CAO, and after CAR. The left ventricle was cut into small pieces(0.19±0.01 g) and separated into non-ischemic (n=912), or area at risk, which contained infarcted (n=182) or salvaged (n=56) samples. Analysis revealed infarcted tissue had higher pre-CAO myocardial blood flow than salvaged tissue(0.93±0.02 vs 0.66±0.04 ml/min/g, P < 0.01). Conversely, infarcted tissue had lower blood flow at 5 min after CAR than salvaged tissue (0.99±0.05 vs 1.34±0.11 ml/min/g, P < 0.01). Thus, spatial heterogeneity of myocardial blood flow in conscious pigs, assessed either prior to CAO or early after CAR, can predict myocardium salvaged by CAR.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997
Externally publishedYes

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Coronary Occlusion
Reperfusion
Coronary Vessels
Blood
Necrosis
Swine
Tissue
Catheters
Microspheres
Myocardial Reperfusion
Spatial Analysis
Papio
Heart Ventricles
Myocardium

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

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title = "Spatial heterogeneity of myocardial blood flow predicts necrosis both prior to coronary occlusion and after reperfusion in conscious pigs",
abstract = "Myocardial blood flow may average 1.0 ml/min/g, but actually ranges from approximately 0.2-2.0 ml/min/g in different myocardial samples due to spatial heterogeneity. We recently observed that the spatial heterogeneity could predict necrosis in baboons with coronary artery occlusion (CAO) and reperfusion (CAR). The goal of this study was to determine if necrosis could be predicted using spatial heterogeneity analyses either prior to CAO or early after CAR in conscious pigs. Accordingly, the effects of CAO for 90 minutes followed by 4-7 days of CAR were examined in 6 conscious pigs instrumented with aortic, left atrial catheters and CA occluders. Myocardial blood flow was measured by radioactive microspheres before CAO, during CAO, and after CAR. The left ventricle was cut into small pieces(0.19±0.01 g) and separated into non-ischemic (n=912), or area at risk, which contained infarcted (n=182) or salvaged (n=56) samples. Analysis revealed infarcted tissue had higher pre-CAO myocardial blood flow than salvaged tissue(0.93±0.02 vs 0.66±0.04 ml/min/g, P < 0.01). Conversely, infarcted tissue had lower blood flow at 5 min after CAR than salvaged tissue (0.99±0.05 vs 1.34±0.11 ml/min/g, P < 0.01). Thus, spatial heterogeneity of myocardial blood flow in conscious pigs, assessed either prior to CAO or early after CAR, can predict myocardium salvaged by CAR.",
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Spatial heterogeneity of myocardial blood flow predicts necrosis both prior to coronary occlusion and after reperfusion in conscious pigs. / Huang, C. H.; Shen, Y. T.; Kim, S. J.; Ghaleh, B.; Kudej, R.; Vatner, Stephen.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997.

Research output: Contribution to journalArticle

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T1 - Spatial heterogeneity of myocardial blood flow predicts necrosis both prior to coronary occlusion and after reperfusion in conscious pigs

AU - Huang, C. H.

AU - Shen, Y. T.

AU - Kim, S. J.

AU - Ghaleh, B.

AU - Kudej, R.

AU - Vatner, Stephen

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Myocardial blood flow may average 1.0 ml/min/g, but actually ranges from approximately 0.2-2.0 ml/min/g in different myocardial samples due to spatial heterogeneity. We recently observed that the spatial heterogeneity could predict necrosis in baboons with coronary artery occlusion (CAO) and reperfusion (CAR). The goal of this study was to determine if necrosis could be predicted using spatial heterogeneity analyses either prior to CAO or early after CAR in conscious pigs. Accordingly, the effects of CAO for 90 minutes followed by 4-7 days of CAR were examined in 6 conscious pigs instrumented with aortic, left atrial catheters and CA occluders. Myocardial blood flow was measured by radioactive microspheres before CAO, during CAO, and after CAR. The left ventricle was cut into small pieces(0.19±0.01 g) and separated into non-ischemic (n=912), or area at risk, which contained infarcted (n=182) or salvaged (n=56) samples. Analysis revealed infarcted tissue had higher pre-CAO myocardial blood flow than salvaged tissue(0.93±0.02 vs 0.66±0.04 ml/min/g, P < 0.01). Conversely, infarcted tissue had lower blood flow at 5 min after CAR than salvaged tissue (0.99±0.05 vs 1.34±0.11 ml/min/g, P < 0.01). Thus, spatial heterogeneity of myocardial blood flow in conscious pigs, assessed either prior to CAO or early after CAR, can predict myocardium salvaged by CAR.

AB - Myocardial blood flow may average 1.0 ml/min/g, but actually ranges from approximately 0.2-2.0 ml/min/g in different myocardial samples due to spatial heterogeneity. We recently observed that the spatial heterogeneity could predict necrosis in baboons with coronary artery occlusion (CAO) and reperfusion (CAR). The goal of this study was to determine if necrosis could be predicted using spatial heterogeneity analyses either prior to CAO or early after CAR in conscious pigs. Accordingly, the effects of CAO for 90 minutes followed by 4-7 days of CAR were examined in 6 conscious pigs instrumented with aortic, left atrial catheters and CA occluders. Myocardial blood flow was measured by radioactive microspheres before CAO, during CAO, and after CAR. The left ventricle was cut into small pieces(0.19±0.01 g) and separated into non-ischemic (n=912), or area at risk, which contained infarcted (n=182) or salvaged (n=56) samples. Analysis revealed infarcted tissue had higher pre-CAO myocardial blood flow than salvaged tissue(0.93±0.02 vs 0.66±0.04 ml/min/g, P < 0.01). Conversely, infarcted tissue had lower blood flow at 5 min after CAR than salvaged tissue (0.99±0.05 vs 1.34±0.11 ml/min/g, P < 0.01). Thus, spatial heterogeneity of myocardial blood flow in conscious pigs, assessed either prior to CAO or early after CAR, can predict myocardium salvaged by CAR.

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