@inbook{29e0b394d15a43208d34cd6b8cde635c,
title = "Spatial organization of enzyme cascade on a DNA origami nanostructure",
abstract = "Self-assembled DNA nanostructures hold great promise to organize multi-enzyme systems with the precise control of the geometric arrangements. Enzymes modified with single-stranded DNA anchors are assembled onto the DNA origami tiles by hybridizing with the corresponding complementary strands displayed on the surface of the DNA nanostructures. Here, we describe a protocol of assembling a two- enzyme cascade on a discrete, rectangular DNA origami tile, where the distance between enzymes is precisely controlled for investigating the distance-dependent cascade activities.",
keywords = "AFM, DNA origami, Denaturing PAGE, Enzyme cascade, Protein-DNA conjugation",
author = "Jinglin Fu and Tianran Li",
note = "Funding Information: This work is supported by an Army Research Office MURI subaward to J.F. (parent award: W911NF-12-1-0420), an Army Research Office Young Investigator award (W911NF-14-1-0434) to J.F., and a Cottrell College Science Award to J.F. Authors are grateful to Dr. Ting Zhang for proofreading the manuscript. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6454-3_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--164",
booktitle = "Methods in Molecular Biology",
}