Spatially selective heteronuclear multiple-quantum coherence spectroscopy for biomolecular NMR studies

Bharathwaj Sathyamoorthy, David M. Parish, Gaetano T. Montelione, Rong Xiao, Thomas Szyperski

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Spatially selective heteronuclear multiple-quantum coherence (SS HMQC) NMR spectroscopy is developed for solution studies of proteins. Due to "time-staggered" acquisitioning of free induction decays (FIDs) in different slices, SS HMQC allows one to use long delays for longitudinal nuclear spin relaxation at high repetition rates. To also achieve high intrinsic sensitivity, SS HMQC is implemented by combining a single spatially selective 1H excitation pulse with nonselective 1H 180° pulses. High-quality spectra were obtained within 66 s for a 7.6 kDa uniformly 13C,15N-labeled protein, and within 45 and 90 s for, respectively, two proteins with molecular weights of 7.5 and 43 kDa, which were uniformly 2H,13C,15N-labeled, except for having protonated methyl groups of isoleucine, leucine and valine residues. Expect longer delays: Spatially selective (SS) HMQC NMR spectroscopy is presented for solution studies of proteins. Using SS HMQC allows one to employ long delays for longitudinal nuclear spin relaxation at high repetition rates for acquisition of free induction decays. This technique is applied to uniformly 13C,15N-labeled and uniformly 2H, 13C,15N-labeled (but methyl group protonated) proteins with molecular weights of 7.5 and 43 kDa.

Original languageEnglish (US)
Pages (from-to)1872-1879
Number of pages8
JournalChemPhysChem
Volume15
Issue number9
DOIs
StatePublished - Jun 23 2014

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

Keywords

  • HMQC
  • flip-back pulses
  • rapid data acquisition
  • spatially selective NMR
  • time-staggered data acquisition

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