Species-Specific Activity of Rat Recombinant Interleukin-1β

Changlu Liu, Yuchen Bai, Doina Ganea, Ronald P. Hart, Changlu Liu

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have constructed expression clones for mature rat interleukin-1β (IL-1β). Using these clones, rat recombinant IL-1β (rrIL-1β) was prepared and purified by a single-step cation-exchange chromatography method. In addition, synthetic rrIL-1β mRNA was transcribed and used to synthesize radiolabeled 35S-rrIL-1β for binding studies. The rrIL-1β produced was demonstrated to have IL-1 bioactivity in a mouse thymocyte proliferation assay, in which it could be blocked by inclusion of human recombinant IL-1 receptor antagonist (hrIL-1Ra). Similarly, rrIL-1β induced LIF mRNA expression in cultured rat sympathetic ganglia. In each case, the maximal dose of rrIL-1β was similar to that of human IL-1β. However, the peak response in rat tissue was twofold higher with rat IL-1β than with human IL-1β. The expression of rrIL-1β provides a useful tool for studying IL-1 receptors in rat, in which human and mouse IL-1β have been found to bind poorly. Species-specific activity of rat IL-1β may correlate with compensatory ionic properties of rat IL-1 receptor.

Original languageEnglish (US)
Pages (from-to)985-992
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number11
DOIs
StatePublished - Nov 1995

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

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