Specific 1,25-dihydroxyvitamin D3 binding sites in choroid plexus

Marian R. Walters, Christine T. Fischette, Carole Fetzer, Byron May, Paul C. Riggle, M. Tibaldo-Bongiorno, Sylvia Christakos

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17 Scopus citations


Quantitative autoradiographic analysis of [3H]1,25-dihydroxyvitamin D3 (1,25(OH)2D3) binding in vitamin D deficient mice provided evidence for high levels of specific binding in choroid plexus and, to a lesser extent, ventral hippocampus. Sucrose gradient analysis yielded a 3-4S peak of specific [3H]1,25(OH)2D3 binding in bovine choroid plexus, but not amygdala or hippocampus. Scatchard analysis of [3H]1,25(OH)2D3 binding in bovine choroid plexus yielded KD = 0.23 ± 0.06 nM and Nmax = 43.5 ± 0 fmol/g tissue (n = 5). This result indicates the presence of significant receptor-like [3]H]1,25(OH)2D3 binding sites in the choroid plexus and, thus suggests roles for this hormone in regulating the entry of calcium into the brain and/or in the central regulation of calcium homeostatis.

Original languageEnglish (US)
Pages (from-to)309-311
Number of pages3
JournalEuropean Journal of Pharmacology
Issue number2
StatePublished - Mar 24 1992

All Science Journal Classification (ASJC) codes

  • Pharmacology


  • 1,25-Dihydroxyvitamin D binding
  • 1,25-Dihydroxyvitamin receptors
  • Ca
  • Choroid plexus


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