Specific in vitro activation of Ca,Mg-ATPase by vitamin d-dependent rat renal calcium binding protein (calbindin D28K)

Douglas W. Morgan; Ann F. Welton; Alison E. Heick; Sylvia Christakos

doi:10.1016/S0006-291X(86)80531-9

Specific in vitro activation of Ca,Mg-ATPase by vitamin d-dependent rat renal calcium binding protein (calbindin D_28K)

Douglas W. Morgan, Ann F. Welton, Alison E. Heick, Sylvia Christakos

Research output: Contribution to journal › Article › peer-review

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Abstract

The vitamin D-dependent, calcium-binding protein from rat kidney, calbindin D_28k (renal CaBP) specifically stimulates Ca,Mg-ATPase activity of human erythrocyte plasma membranes in a dose-dependent, calcium-sensitive manner. This stimulation was about two-fold compared to a three-fold stimulation by calmodulin. The effect was specific since other calcium-binding proteins and low molecular weight proteins did not stimulate Ca,Mg-ATPase activity. Renal CaBP did not stimulate cyclic nucleotide phosphodiesterase at concentrations greater than those which stimulated Ca,Mg-ATPase activity. This is the first report of a specific in vitro effect of renal CaBP on an enzyme system.

Original language	English (US)
Pages (from-to)	547-553
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	138
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80531-9
State	Published - Jul 31 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(86)80531-9

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title = "Specific in vitro activation of Ca,Mg-ATPase by vitamin d-dependent rat renal calcium binding protein (calbindin D28K)",

abstract = "The vitamin D-dependent, calcium-binding protein from rat kidney, calbindin D28k (renal CaBP) specifically stimulates Ca,Mg-ATPase activity of human erythrocyte plasma membranes in a dose-dependent, calcium-sensitive manner. This stimulation was about two-fold compared to a three-fold stimulation by calmodulin. The effect was specific since other calcium-binding proteins and low molecular weight proteins did not stimulate Ca,Mg-ATPase activity. Renal CaBP did not stimulate cyclic nucleotide phosphodiesterase at concentrations greater than those which stimulated Ca,Mg-ATPase activity. This is the first report of a specific in vitro effect of renal CaBP on an enzyme system.",

author = "Morgan, {Douglas W.} and Welton, {Ann F.} and Heick, {Alison E.} and Sylvia Christakos",

note = "Funding Information: This work was supported in part by Hoffmann-La Roche, Inc. and ~ Grant PCM-8103421 and NIH Grant NS-20270. SC is a recipient of a Research Career Development Award from NIADDK (AM-01200). To whom reprint request should be addressed.",

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language = "English (US)",

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AU - Morgan, Douglas W.

AU - Welton, Ann F.

AU - Heick, Alison E.

AU - Christakos, Sylvia

N1 - Funding Information: This work was supported in part by Hoffmann-La Roche, Inc. and ~ Grant PCM-8103421 and NIH Grant NS-20270. SC is a recipient of a Research Career Development Award from NIADDK (AM-01200). To whom reprint request should be addressed.

PY - 1986/7/31

Y1 - 1986/7/31

N2 - The vitamin D-dependent, calcium-binding protein from rat kidney, calbindin D28k (renal CaBP) specifically stimulates Ca,Mg-ATPase activity of human erythrocyte plasma membranes in a dose-dependent, calcium-sensitive manner. This stimulation was about two-fold compared to a three-fold stimulation by calmodulin. The effect was specific since other calcium-binding proteins and low molecular weight proteins did not stimulate Ca,Mg-ATPase activity. Renal CaBP did not stimulate cyclic nucleotide phosphodiesterase at concentrations greater than those which stimulated Ca,Mg-ATPase activity. This is the first report of a specific in vitro effect of renal CaBP on an enzyme system.

AB - The vitamin D-dependent, calcium-binding protein from rat kidney, calbindin D28k (renal CaBP) specifically stimulates Ca,Mg-ATPase activity of human erythrocyte plasma membranes in a dose-dependent, calcium-sensitive manner. This stimulation was about two-fold compared to a three-fold stimulation by calmodulin. The effect was specific since other calcium-binding proteins and low molecular weight proteins did not stimulate Ca,Mg-ATPase activity. Renal CaBP did not stimulate cyclic nucleotide phosphodiesterase at concentrations greater than those which stimulated Ca,Mg-ATPase activity. This is the first report of a specific in vitro effect of renal CaBP on an enzyme system.

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Specific in vitro activation of Ca,Mg-ATPase by vitamin d-dependent rat renal calcium binding protein (calbindin D_28K)

Abstract

All Science Journal Classification (ASJC) codes

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