Specific in vitro activation of Ca,Mg-ATPase by vitamin d-dependent rat renal calcium binding protein (calbindin D28K)

Douglas W. Morgan, Ann F. Welton, Alison E. Heick, Sylvia Christakos

Research output: Contribution to journalArticle

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Abstract

The vitamin D-dependent, calcium-binding protein from rat kidney, calbindin D28k (renal CaBP) specifically stimulates Ca,Mg-ATPase activity of human erythrocyte plasma membranes in a dose-dependent, calcium-sensitive manner. This stimulation was about two-fold compared to a three-fold stimulation by calmodulin. The effect was specific since other calcium-binding proteins and low molecular weight proteins did not stimulate Ca,Mg-ATPase activity. Renal CaBP did not stimulate cyclic nucleotide phosphodiesterase at concentrations greater than those which stimulated Ca,Mg-ATPase activity. This is the first report of a specific in vitro effect of renal CaBP on an enzyme system.

Original languageEnglish (US)
Pages (from-to)547-553
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number2
DOIs
StatePublished - Jul 31 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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