Spoligotyping of mycobacterium tuberculosis complex isolates by use of ligation-based amplification and melting curve analysis

Xiaohong Zeng, Hui Li, Rongrong Zheng, Natalia Kurepina, Barry N. Kreiswirth, Xilin Zhao, Ye Xu, Qingge Lia

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

Original languageEnglish (US)
Pages (from-to)2384-2387
Number of pages4
JournalJournal of clinical microbiology
Volume54
Issue number9
DOIs
StatePublished - Sep 2016

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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