Ssu72 is an RNA polymerase II CTD phosphatase

Shankarling Krishnamurthy, Xiaoyuan He, Mariela Reyes-Reyes, Claire Moore, Michael Hampsey

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

Phosphorylation of serine-2 (S2) and serine-5 (S5) of the C-terminal domain (CTD) of RNA polymerase II (RNAP II) is a dynamic process that regulates the transcription cycle and coordinates recruitment of RNA processing factors. The Fcp1 CTD phosphatase catalyzes dephosphorylation of S2-P. Here, we report that Ssu72, a component of the yeast cleavage/polyadenylation factor (CPF) complex, is a CTD phosphatase with specificity for S5-P. Ssu72 catalyzes CTD S5-P dephosphorylation in association with the Pta1 component of the CPF complex, although its essential role in 3′ end processing is independent of catalytic activity. Depletion of Ssu72 impairs transcription in vitro, and this defect can be rescued by recombinant, catalytically active Ssu72. We propose that Ssu72 has a dual role in transcription, one as a CTD S5-P phosphatase that regenerates the initiation-competent, hypophosphorylated form of RNAP II and the other as a factor necessary for cleavage of pre-mRNA and efficient transcription termination.

Original languageEnglish (US)
Pages (from-to)387-394
Number of pages8
JournalMolecular cell
Volume14
Issue number3
DOIs
StatePublished - May 7 2004

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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