Stable genetic transformation of Eschscholzia californica expressing synthetic green fluorescent proteins

J. Lee, H. Pedersen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

An efficient protocol is described for the stable genetic transformation of Eschscholzia californica (California poppy) using Agrobacterium tumefaciens as a vector. We have employed the disarmed A. tumefaciens LBA4404 encoding a synthetic green fluorescent protein reporter gene that is further controlled by an enhanced cauliflower mosaic virus 35S promoter. Stably transformed E. californica cells appear 3 weeks after initial cocultivation of A. tumefaciens with poppy leaves, stems, or roots. Transformed poppy calli were visualized by exposure to long-wave UV or blue light and analyzed in detail by fluorescent microscopy and laser-scanning microscopy. Moreover, green fluorescent calli have been maintained through continual subculture and grow well either on Gamborg's B5 agarose or liquid medium.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalBiotechnology Progress
Volume17
Issue number2
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology

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