Stable isotope analysis of breath using the optogalvanic effect

Daniel Murnick, M. J. Colgan, H. P. Lie, D. Stoneback

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A new technique based on the optogalvanic effect has been developed for the measurement of stable isotope ratios in the carbon dioxide of exhaled breath. Data obtained before and after ingestion of harmless stable isotope labeled compounds, metabolized to carbon dioxide, can be used for sensitive noninvasive diagnostics of various disease conditions. The technique uses the specificity of laser resonance spectroscopy and achieves sensitivity and accuracy typical of sophisticated isotope ratio mass spectrometers. Using fixed frequency carbon dioxide lasers, 13C/ 12C ratios can be determined with a precision of 2 ppm with 100 second averaging times. Multiple samples can be analyzed simultaneously providing real time continuous calibration. In a first application, analysis of 13C/ 12C ratios in exhaled human breath after ingestion of 13C labeled urea is being developed as a diagnostic for the bacterium H-pylori, known to be the causative agent for most peptic and duodenal ulcers.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsDaniel L. Farkas, Robert C. Leif, Alexander V. Priezzhev, Toshimitsu Asakura, Bruce J. Tromberg
Pages454-463
Number of pages10
Volume2678
StatePublished - Jan 1 1996
EventOptical Diagnostics of Living Cells and Biofluids - San Jose, CA, USA
Duration: Jan 28 1996Feb 1 1996

Other

OtherOptical Diagnostics of Living Cells and Biofluids
CitySan Jose, CA, USA
Period1/28/962/1/96

Fingerprint

Isotopes
Carbon dioxide
Carbon dioxide lasers
Mass spectrometers
Urea
Bacteria
Spectroscopy
Calibration
Lasers

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Murnick, D., Colgan, M. J., Lie, H. P., & Stoneback, D. (1996). Stable isotope analysis of breath using the optogalvanic effect. In D. L. Farkas, R. C. Leif, A. V. Priezzhev, T. Asakura, & B. J. Tromberg (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2678, pp. 454-463)
Murnick, Daniel ; Colgan, M. J. ; Lie, H. P. ; Stoneback, D. / Stable isotope analysis of breath using the optogalvanic effect. Proceedings of SPIE - The International Society for Optical Engineering. editor / Daniel L. Farkas ; Robert C. Leif ; Alexander V. Priezzhev ; Toshimitsu Asakura ; Bruce J. Tromberg. Vol. 2678 1996. pp. 454-463
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Murnick, D, Colgan, MJ, Lie, HP & Stoneback, D 1996, Stable isotope analysis of breath using the optogalvanic effect. in DL Farkas, RC Leif, AV Priezzhev, T Asakura & BJ Tromberg (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2678, pp. 454-463, Optical Diagnostics of Living Cells and Biofluids, San Jose, CA, USA, 1/28/96.

Stable isotope analysis of breath using the optogalvanic effect. / Murnick, Daniel; Colgan, M. J.; Lie, H. P.; Stoneback, D.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg. Vol. 2678 1996. p. 454-463.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Murnick D, Colgan MJ, Lie HP, Stoneback D. Stable isotope analysis of breath using the optogalvanic effect. In Farkas DL, Leif RC, Priezzhev AV, Asakura T, Tromberg BJ, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2678. 1996. p. 454-463