Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

Rahul Chib; Sunil Shah; Zygmunt Gryczynski; Rafal Fudala; Julian Borejdo; Bogumil Zelent; Maria G. Corradini; Richard D. Ludescher; Ignacy Gryczynski

doi:10.1088/0957-0233/27/2/027001

Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

Rahul Chib, Sunil Shah, Zygmunt Gryczynski, Rafal Fudala, Julian Borejdo, Bogumil Zelent, Maria G. Corradini, Richard D. Ludescher, Ignacy Gryczynski

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6 Scopus citations

Abstract

Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

Original language	English (US)
Article number	027001
Journal	Measurement Science and Technology
Volume	27
Issue number	2
DOIs	https://doi.org/10.1088/0957-0233/27/2/027001
State	Published - Dec 29 2015

All Science Journal Classification (ASJC) codes

Instrumentation
Engineering (miscellaneous)
Applied Mathematics

Keywords

allura red
fluorescence lifetime
instrument response function
lifetime standard
scatterer substitute

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10.1088/0957-0233/27/2/027001

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title = "Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared",

abstract = "Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.",

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T1 - Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

AU - Chib, Rahul

AU - Shah, Sunil

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

AU - Borejdo, Julian

AU - Zelent, Bogumil

AU - Corradini, Maria G.

AU - Ludescher, Richard D.

AU - Gryczynski, Ignacy

PY - 2015/12/29

Y1 - 2015/12/29

N2 - Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

AB - Allura red (AR) fluorophore, a common dye in the food industry, displays a broad emission spectrum in water (visible-to-near infrared region of the electromagnetic spectrum) and has a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength. We examined time responses of AR fluorescence across emission wavelengths from 550 nm to 750 nm and found that it is an ideal candidate for impulse response functions in fluorescence lifetime measurements.

KW - allura red

KW - fluorescence lifetime

KW - instrument response function

KW - lifetime standard

KW - scatterer substitute

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DO - 10.1088/0957-0233/27/2/027001

M3 - Article

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SN - 0957-0233

VL - 27

JO - Measurement Science and Technology

JF - Measurement Science and Technology

IS - 2

M1 - 027001

ER -

Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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