@inbook{ce533baa3dae440cb1362d44dc26c705,
title = "Static and kinetic site-specific protein-DNA photocrosslinking: Analysis of bacterial transcription initiation complexes",
abstract = "Static site-specific protein-DNA photocrosslinking permits identification of protein-DNA interactions within multiprotein-DNA complexes. Kinetic site-specific protein-DNA photocrosslinking - involving rapid-quench-flow mixing and pulsed-laser irradiation - permits elucidation of pathways and kinetics of formation of protein-DNA interactions within multiprotein-DNA complexes. We present detailed protocols for application of static and kinetic site-specific protein-DNA photocrosslinking to bacterial transcription initiation complexes.",
keywords = "Kinetics, Open complex, Phenyl-azide photoactivatible crosslinking agent, Phosphate, Phosphorothioate, Promoter, Protein-DNA interaction, Pulsed-laser UV irradiations, Quench-flow rapid mixing, RNA polymerase, Structure, Transcription initiation",
author = "Nikolai Naryshkin and Sergei Druzhinin and Andrei Revyakin and Younggyu Kim and Vladimir Mekler and Ebright, {Richard H.}",
year = "2009",
doi = "10.1007/978-1-60327-015-1_25",
language = "English (US)",
isbn = "9781603270144",
series = "Methods in Molecular Biology",
pages = "403--437",
editor = "Tom Moss and Benoit Leblanc",
booktitle = "DNA-Protein Interactions",
}