Steinernema glaseri surface coat protein suppresses the immune response of Popillia japonica (Coleoptera: Scarabaeidae) larvae

Yi Wang, Randy Gaugler

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The host immune response is a key obstacle to entomopathogenic nematodes in making the transition from the free-living state to parasitism. The entomopathogenic nematode Steinernema glaseri has evolved mechanisms to evade immune encapsulation in larvae of the Japanese beetle, Popillia japonica. Host intrahemocoelic injection tests show that live axenic nematodes of S. glaseri not only avoid host melanotic encapsulation but also protect dead nematodes injected after the live ones. This result indicates that the nematodes release anti-immune factor(s). We extracted the nematode surface coat proteins and found that at least one protein (SCP3a) from the S. glaseri surface coat can suppress the host immune system. This suppression protects unrelated nematode species from encapsulation and latex beads from phagocytosis. We conclude that S. glaseri uses an anti-immune protein to defeat the host immune system, thereby protecting itself from encapsulation. Presumably its symbiotic bacteria are similarly protected from phagocytosis.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalBiological Control
Volume14
Issue number1
DOIs
StatePublished - Jan 1999

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Keywords

  • Entomopathogenic nematode
  • Immune suppression
  • Japanese beetle
  • Melanotic encapsulation
  • Steinernema glaseri
  • Surface coat protein

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