Stepwise functionalization of ZnO nanotips with DNA

Olena Taratula, Elena Galoppini, Richard Mendelsohn, Pavel Ivanoff Reyes, Zheng Zhang, Ziqing Duan, Jian Zhong, Yicheng Lu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A surface functionalization methodology for the development of ZnO nanotips biosensors that can be integrated with microelectronics was developed. Two types of long chain carboxylic acids linkers were employed for the functionalization of 0.5 μm thick MOCVD-grown ZnO nanotip films with single-stranded DNA (ssDNA), followed by hybridization with complementary ssDNA tagged with fluorescein. The ZnO functionalization strategy was developed for the fabrication of ZnO nanotips-linker-biomolecule films integrated with bulk acoustic wave (BAW) biosensors, and it involved three main steps. First, 16-(2-pyridyldithiol)hexadecanoic acid or N-(15-carboxypentadecanoyloxy) succinimide, both bifunctional C16 carboxylic acids, were bound to ZnO nanotip films through the COOH group, leaving at the opposite end of the alkyl chain a thiol group protected as a 2-pyridyl disulfide, or a carboxylic group protected as a N-succinimide, respectively. In the second step, ssDNA was covalently linked to each type of ZnO-linker film: the 2-pyridyl disulfide end group was substituted with 16 bases 5′-thiol-modified DNA (SH-ssDNA), and the N-succinimide ester end group was substituted with 16 bases 5′-amino- modified DNA (NH 2-ssDNA). In the third step, the DNA-functionalized ZnO nanotip films were hybridized with complementary 5′-fluorescein ssDNA. The surface-modified ZnO nanotip films were characterized after each step by FT-IR-ATR, fluorescence emission spectroscopy, and fluorescence microscopy. This functionalization approach allows sequential reactions on the surface and, in principle, can be extended to numerous other molecules and biomolecules.

Original languageEnglish (US)
Pages (from-to)2107-2113
Number of pages7
JournalLangmuir
Volume25
Issue number4
DOIs
StatePublished - Feb 17 2009

Fingerprint

Nanotips
Single-Stranded DNA
DNA
deoxyribonucleic acid
succinimides
Biomolecules
Carboxylic Acids
Fluorescein
Sulfhydryl Compounds
Biosensors
Disulfides
disulfides
Carboxylic acids
bioinstrumentation
thiols
carboxylic acids
Palmitic Acid
Fluorescence microscopy
Fluorescence spectroscopy
Emission spectroscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Taratula, Olena ; Galoppini, Elena ; Mendelsohn, Richard ; Reyes, Pavel Ivanoff ; Zhang, Zheng ; Duan, Ziqing ; Zhong, Jian ; Lu, Yicheng. / Stepwise functionalization of ZnO nanotips with DNA. In: Langmuir. 2009 ; Vol. 25, No. 4. pp. 2107-2113.
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abstract = "A surface functionalization methodology for the development of ZnO nanotips biosensors that can be integrated with microelectronics was developed. Two types of long chain carboxylic acids linkers were employed for the functionalization of 0.5 μm thick MOCVD-grown ZnO nanotip films with single-stranded DNA (ssDNA), followed by hybridization with complementary ssDNA tagged with fluorescein. The ZnO functionalization strategy was developed for the fabrication of ZnO nanotips-linker-biomolecule films integrated with bulk acoustic wave (BAW) biosensors, and it involved three main steps. First, 16-(2-pyridyldithiol)hexadecanoic acid or N-(15-carboxypentadecanoyloxy) succinimide, both bifunctional C16 carboxylic acids, were bound to ZnO nanotip films through the COOH group, leaving at the opposite end of the alkyl chain a thiol group protected as a 2-pyridyl disulfide, or a carboxylic group protected as a N-succinimide, respectively. In the second step, ssDNA was covalently linked to each type of ZnO-linker film: the 2-pyridyl disulfide end group was substituted with 16 bases 5′-thiol-modified DNA (SH-ssDNA), and the N-succinimide ester end group was substituted with 16 bases 5′-amino- modified DNA (NH 2-ssDNA). In the third step, the DNA-functionalized ZnO nanotip films were hybridized with complementary 5′-fluorescein ssDNA. The surface-modified ZnO nanotip films were characterized after each step by FT-IR-ATR, fluorescence emission spectroscopy, and fluorescence microscopy. This functionalization approach allows sequential reactions on the surface and, in principle, can be extended to numerous other molecules and biomolecules.",
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Taratula, O, Galoppini, E, Mendelsohn, R, Reyes, PI, Zhang, Z, Duan, Z, Zhong, J & Lu, Y 2009, 'Stepwise functionalization of ZnO nanotips with DNA', Langmuir, vol. 25, no. 4, pp. 2107-2113. https://doi.org/10.1021/la8026946

Stepwise functionalization of ZnO nanotips with DNA. / Taratula, Olena; Galoppini, Elena; Mendelsohn, Richard; Reyes, Pavel Ivanoff; Zhang, Zheng; Duan, Ziqing; Zhong, Jian; Lu, Yicheng.

In: Langmuir, Vol. 25, No. 4, 17.02.2009, p. 2107-2113.

Research output: Contribution to journalArticle

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AU - Taratula, Olena

AU - Galoppini, Elena

AU - Mendelsohn, Richard

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