Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis

Dhirendra K. Simanshu, Yoshihiro Yamaguchi, Jung Ho Park, Masayori Inouye, Dinshaw J. Patel

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

MazF is an mRNA interferase, which, upon activation during stress conditions, cleaves mRNAs in a sequence-specific manner, resulting in cellular growth arrest. During normal growth conditions, the MazF toxin is inactivated through binding to its cognate antitoxin, MazE. How MazF specifically recognizes its mRNA target and carries out cleavage and how the formation of the MazE-MazF complex inactivates MazF remain unclear. We present crystal structures of MazF in complex with mRNA substrate and antitoxin MazE in Bacillus subtilis. The structure of MazF in complex with uncleavable UUdUACAUAA RNA substrate defines the molecular basis underlying the sequence-specific recognition of UACAU and the role of residues involved in the cleavage through site-specific mutational studies. The structure of the heterohexameric (MazF)2-(MazE)2-(MazF)2 complex in Bacillus subtilis, supplemented by mutational data, demonstrates that the positioning of the C-terminal helical segment of MazE within the RNA-binding channel of the MazF dimer prevents mRNA binding and cleavage by MazF.

Original languageEnglish (US)
Pages (from-to)447-458
Number of pages12
JournalMolecular cell
Volume52
Issue number3
DOIs
StatePublished - Nov 7 2013

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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