Structural features of carbohydrate chains in human salivary mucins

B. L. Slomiany, V. L.N. Murty, A. Slomiany

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

1. 1. The structure of carbohydrate chains in the low and high molecular weight mucus glycoprotein forms from submandibular-sublingual saliva of individuals with blood group B was investigated. 2. 2. Alkaline borohydride reductive cleavage of the glycoproteins yielded in each case a population of neutral (55%) and acidic (45%) oligosaccharide alditols ranging in size from 3 to 16 sugar units. 3. 3. The predominant neutral oligosaccharides in both glycoprotein forms consisted of 16 and 15 sugar units arranged in triantennary fashion, and carried blood group B and I antigenic determinants. 4. 4. Three of the oligosaccharides in each glycoprotein contained sialic acid and ranged in size from 3 to 12 sugar units. In two oligosaccharides sialic acid was linked to C3 of galactose and in one to C6 of N-acetylgalactosamine. The sulfated oligosaccharide in both glycoproteins was identified as a pentasaccharide with the sulfate ester group at C6 of N-acetylglucosamine. 5. 5. The results demonstrate that contrary to the earlier view the low and high molecular weight mucus glycoprotein forms of human saliva contain identical carbohydrate chains.

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalInternational Journal of Biochemistry
Volume25
Issue number2
DOIs
StatePublished - Feb 1993

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Mucins
Oligosaccharides
Carbohydrates
Glycoproteins
Sugars
N-Acetylneuraminic Acid
Blood Group Antigens
Saliva
Molecular Weight
Molecular weight
Sugar Alcohols
Acetylgalactosamine
Borohydrides
Acetylglucosamine
Galactose
Sulfates
Epitopes
Esters
Population
gastric mucus glycoproteins

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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title = "Structural features of carbohydrate chains in human salivary mucins",
abstract = "1. 1. The structure of carbohydrate chains in the low and high molecular weight mucus glycoprotein forms from submandibular-sublingual saliva of individuals with blood group B was investigated. 2. 2. Alkaline borohydride reductive cleavage of the glycoproteins yielded in each case a population of neutral (55{\%}) and acidic (45{\%}) oligosaccharide alditols ranging in size from 3 to 16 sugar units. 3. 3. The predominant neutral oligosaccharides in both glycoprotein forms consisted of 16 and 15 sugar units arranged in triantennary fashion, and carried blood group B and I antigenic determinants. 4. 4. Three of the oligosaccharides in each glycoprotein contained sialic acid and ranged in size from 3 to 12 sugar units. In two oligosaccharides sialic acid was linked to C3 of galactose and in one to C6 of N-acetylgalactosamine. The sulfated oligosaccharide in both glycoproteins was identified as a pentasaccharide with the sulfate ester group at C6 of N-acetylglucosamine. 5. 5. The results demonstrate that contrary to the earlier view the low and high molecular weight mucus glycoprotein forms of human saliva contain identical carbohydrate chains.",
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Structural features of carbohydrate chains in human salivary mucins. / Slomiany, B. L.; Murty, V. L.N.; Slomiany, A.

In: International Journal of Biochemistry, Vol. 25, No. 2, 02.1993, p. 259-265.

Research output: Contribution to journalArticle

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AB - 1. 1. The structure of carbohydrate chains in the low and high molecular weight mucus glycoprotein forms from submandibular-sublingual saliva of individuals with blood group B was investigated. 2. 2. Alkaline borohydride reductive cleavage of the glycoproteins yielded in each case a population of neutral (55%) and acidic (45%) oligosaccharide alditols ranging in size from 3 to 16 sugar units. 3. 3. The predominant neutral oligosaccharides in both glycoprotein forms consisted of 16 and 15 sugar units arranged in triantennary fashion, and carried blood group B and I antigenic determinants. 4. 4. Three of the oligosaccharides in each glycoprotein contained sialic acid and ranged in size from 3 to 12 sugar units. In two oligosaccharides sialic acid was linked to C3 of galactose and in one to C6 of N-acetylgalactosamine. The sulfated oligosaccharide in both glycoproteins was identified as a pentasaccharide with the sulfate ester group at C6 of N-acetylglucosamine. 5. 5. The results demonstrate that contrary to the earlier view the low and high molecular weight mucus glycoprotein forms of human saliva contain identical carbohydrate chains.

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