We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RP0). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RP0, permit construction of a detailed model for the structure of RP0, and permit analysis of effects of a transcriptional activator on the structure of RP0.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)