Structural organization of the RNA polymerase-promoter open complex

Nikolai Naryshkin, Andrey Revyakin, Younggyu Kim, Vladimir Mekler, Richard H. Ebright

Research output: Contribution to journalArticlepeer-review

155 Scopus citations


We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RP0). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RP0, permit construction of a detailed model for the structure of RP0, and permit analysis of effects of a transcriptional activator on the structure of RP0.

Original languageEnglish (US)
Pages (from-to)601-611
Number of pages11
Issue number6
StatePublished - Jun 9 2000

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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