Structure and characterization of the gene encoding a mouse kappa3-related opioid receptor

Ying Xian Pan; Jin Xu; Gavril W. Pasternak

doi:10.1016/0378-1119(95)00890-X

Structure and characterization of the gene encoding a mouse kappa₃-related opioid receptor

Ying Xian Pan, Jin Xu, Gavril W. Pasternak

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A genomic clone comprising the entire cDNA sequence encoding a mouse kappa₃-related opioid receptor (KOR-3) was isolated by screening a mouse genomic library with a radiolabeled mouse KOR-3 cDNA probe. Sequence analysis of the genomic clone indicates that the KOR-3 gene contains five exons separated by four introns. The transcription start point (tsp) of KOR-3 was mapped by primer extension analysis of RNAs synthesized either in vivo or in vitro. A TATA-box and several potential regulatory elements, including five GRE sites, four NF-E1 binding sites and one MRE site, are present in the 2 kb of 5'-flanking region. A putative poly(A) signal (AATAAA) is found in the 3'-flanking region.

Original language	English (US)
Pages (from-to)	255-260
Number of pages	6
Journal	Gene
Volume	171
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00890-X
State	Published - Jun 1 1996
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

Keywords

Gene structure
KOR-3
Nucleotide sequence
Transcription

Access to Document

10.1016/0378-1119(95)00890-X

Cite this

@article{8cc225a1d60e48899d8e395b05e9dbf9,

title = "Structure and characterization of the gene encoding a mouse kappa3-related opioid receptor",

abstract = "A genomic clone comprising the entire cDNA sequence encoding a mouse kappa3-related opioid receptor (KOR-3) was isolated by screening a mouse genomic library with a radiolabeled mouse KOR-3 cDNA probe. Sequence analysis of the genomic clone indicates that the KOR-3 gene contains five exons separated by four introns. The transcription start point (tsp) of KOR-3 was mapped by primer extension analysis of RNAs synthesized either in vivo or in vitro. A TATA-box and several potential regulatory elements, including five GRE sites, four NF-E1 binding sites and one MRE site, are present in the 2 kb of 5'-flanking region. A putative poly(A) signal (AATAAA) is found in the 3'-flanking region.",

keywords = "Gene structure, KOR-3, Nucleotide sequence, Transcription",

author = "Pan, {Ying Xian} and Jin Xu and Pasternak, {Gavril W.}",

note = "Funding Information: We thank Dr. J. Posner for his support of these studies. This work was supported by a grant to GWP from the National Institute on Drug Abuse (DA02615). Y.-X. P. is supported by a Fellowship from the Aaron Diamond Foundation and GWP by a Research Scientist Award from the National Institute on Drug Abuse (DA00220).",

year = "1996",

month = jun,

day = "1",

doi = "10.1016/0378-1119(95)00890-X",

language = "English (US)",

volume = "171",

pages = "255--260",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Structure and characterization of the gene encoding a mouse kappa3-related opioid receptor

AU - Pan, Ying Xian

AU - Xu, Jin

AU - Pasternak, Gavril W.

N1 - Funding Information: We thank Dr. J. Posner for his support of these studies. This work was supported by a grant to GWP from the National Institute on Drug Abuse (DA02615). Y.-X. P. is supported by a Fellowship from the Aaron Diamond Foundation and GWP by a Research Scientist Award from the National Institute on Drug Abuse (DA00220).

PY - 1996/6/1

Y1 - 1996/6/1

N2 - A genomic clone comprising the entire cDNA sequence encoding a mouse kappa3-related opioid receptor (KOR-3) was isolated by screening a mouse genomic library with a radiolabeled mouse KOR-3 cDNA probe. Sequence analysis of the genomic clone indicates that the KOR-3 gene contains five exons separated by four introns. The transcription start point (tsp) of KOR-3 was mapped by primer extension analysis of RNAs synthesized either in vivo or in vitro. A TATA-box and several potential regulatory elements, including five GRE sites, four NF-E1 binding sites and one MRE site, are present in the 2 kb of 5'-flanking region. A putative poly(A) signal (AATAAA) is found in the 3'-flanking region.

AB - A genomic clone comprising the entire cDNA sequence encoding a mouse kappa3-related opioid receptor (KOR-3) was isolated by screening a mouse genomic library with a radiolabeled mouse KOR-3 cDNA probe. Sequence analysis of the genomic clone indicates that the KOR-3 gene contains five exons separated by four introns. The transcription start point (tsp) of KOR-3 was mapped by primer extension analysis of RNAs synthesized either in vivo or in vitro. A TATA-box and several potential regulatory elements, including five GRE sites, four NF-E1 binding sites and one MRE site, are present in the 2 kb of 5'-flanking region. A putative poly(A) signal (AATAAA) is found in the 3'-flanking region.

KW - Gene structure

KW - KOR-3

KW - Nucleotide sequence

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0029920033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029920033&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(95)00890-X

DO - 10.1016/0378-1119(95)00890-X

M3 - Article

C2 - 8666282

AN - SCOPUS:0029920033

SN - 0378-1119

VL - 171

SP - 255

EP - 260

JO - Gene

JF - Gene

IS - 2

ER -