Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase β′ subunit

Mark Chlenov, Shoko Masuda, Katsuhiko S. Murakami, Vadim Nikiforov, Seth A. Darst, Arkady Mustaev

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The large β and β′ subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in β and β′ characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP β′ subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP β′ subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two β′ insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.

Original languageEnglish (US)
Pages (from-to)138-154
Number of pages17
JournalJournal of molecular biology
Volume353
Issue number1
DOIs
StatePublished - Oct 14 2005
Externally publishedYes

Fingerprint

Bacterial RNA
Insertional Mutagenesis
DNA-Directed RNA Polymerases
Taq Polymerase
Proteins
Nucleic Acids
Sequence Analysis
Escherichia coli
Bacteria

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Keywords

  • RNA polymerase

Cite this

Chlenov, Mark ; Masuda, Shoko ; Murakami, Katsuhiko S. ; Nikiforov, Vadim ; Darst, Seth A. ; Mustaev, Arkady. / Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase β′ subunit. In: Journal of molecular biology. 2005 ; Vol. 353, No. 1. pp. 138-154.
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Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase β′ subunit. / Chlenov, Mark; Masuda, Shoko; Murakami, Katsuhiko S.; Nikiforov, Vadim; Darst, Seth A.; Mustaev, Arkady.

In: Journal of molecular biology, Vol. 353, No. 1, 14.10.2005, p. 138-154.

Research output: Contribution to journalArticle

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