Structure, function, and expression of SEL-1, a negative regulator of LIN-12 and GLP-1 in C. elegans

Barth Grant, Iva Greenwald

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Previous work indicated that sel-1 functions as a negative regulator of lin-12 activity, and predicted that SEL-1 is a secreted or membrane associated protein. In this study, we describe cell ablation experiments that suggest sel-1 mutations elevate lin-12 activity cell autonomously. We also use transgenic approaches to demonstrate that the predicted signal sequence of SEL-1 can direct secretion and is important for function, while a C-terminal hydrophobic region is not required for SEL-1 function. In addition, by analyzing SEL-1 localization using specific antisera we find that SEL-1 is localized intracellularly, with a punctate staining pattern suggestive of membrane bound vesicles. We incorporate these observations, and new information about a related yeast gene, into a proposal for a possible mechanism for SEL-1 function in LIN-12 turnover.

Original languageEnglish (US)
Pages (from-to)637-644
Number of pages8
JournalDevelopment
Volume124
Issue number3
StatePublished - Feb 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Keywords

  • C. elegans
  • Degradation
  • LIN-12
  • Notch
  • SEL-1

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