Structure-independent nucleotide sequence analysis.

D. R. Mills, F. R. Kramer

Research output: Contribution to journalArticlepeer-review

134 Scopus citations

Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

Original languageEnglish (US)
Pages (from-to)2232-2235
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number5
DOIs
StatePublished - May 1979
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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