Structure-independent nucleotide sequence analysis.

D. R. Mills; F. R. Kramer

doi:10.1073/pnas.76.5.2232

Structure-independent nucleotide sequence analysis.

D. R. Mills, F. R. Kramer

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

Original language	English (US)
Pages (from-to)	2232-2235
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	76
Issue number	5
DOIs	https://doi.org/10.1073/pnas.76.5.2232
State	Published - May 1979
Externally published	Yes

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title = "Structure-independent nucleotide sequence analysis.",

abstract = "Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.",

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year = "1979",

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AU - Kramer, F. R.

PY - 1979/5

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N2 - Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

AB - Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

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U2 - 10.1073/pnas.76.5.2232

DO - 10.1073/pnas.76.5.2232

M3 - Article

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AN - SCOPUS:0018470801

VL - 76

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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IS - 5

ER -

Structure-independent nucleotide sequence analysis.

Abstract

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