TY - JOUR
T1 - Structure, mechanism, and regulation of mitochondrial DNA transcription initiation
AU - Basu, Urmimala
AU - Bostwick, Alicia M.
AU - Das, Kalyan
AU - Dittenhafer-Reed, Kristin E.
AU - Patel, Smita S.
N1 - Funding Information:
Funding and additional information—This work was supported by National Institutes of Health Grant R35 GM118086 (to S. S. P.); American Heart Association Grant 16PRE30400001 and a University and Louis Bevier Dissertation Completion Fellowship from Rutgers University (to U. B.); National Science Foundation Grant 1814845 (to K. D. R.); and a KU Leuven start-up grant (to K. D.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2020 Basu et al.
PY - 2020/12/25
Y1 - 2020/12/25
N2 - Mitochondria are specialized compartments that produce requisite ATP to fuel cellular functions and serve as centers of metabolite processing, cellular signaling, and apoptosis. To accomplish these roles, mitochondria rely on the genetic information in their small genome (mitochondrial DNA) and the nucleus. A growing appreciation for mitochondria’s role in a myriad of human diseases, including inherited genetic disorders, degenerative diseases, inflammation, and cancer, has fueled the study of biochemical mechanisms that control mitochondrial function. The mitochondrial transcriptional machinery is different from nuclear machinery. The in vitro reconstituted transcriptional complexes of Saccharomyces cerevisiae (yeast) and humans, aided with high-resolution structures and biochemical characterizations, have provided a deeper understanding of the mechanism and regulation of mitochondrial DNA transcription. In this review, we will discuss recent advances in the structure and mechanism of mitochondrial transcription initiation. We will follow up with recent discoveries and formative findings regarding the regulatory events that control mitochondrial DNA transcription, focusing on those involved in cross-talk between the mitochondria and nucleus.
AB - Mitochondria are specialized compartments that produce requisite ATP to fuel cellular functions and serve as centers of metabolite processing, cellular signaling, and apoptosis. To accomplish these roles, mitochondria rely on the genetic information in their small genome (mitochondrial DNA) and the nucleus. A growing appreciation for mitochondria’s role in a myriad of human diseases, including inherited genetic disorders, degenerative diseases, inflammation, and cancer, has fueled the study of biochemical mechanisms that control mitochondrial function. The mitochondrial transcriptional machinery is different from nuclear machinery. The in vitro reconstituted transcriptional complexes of Saccharomyces cerevisiae (yeast) and humans, aided with high-resolution structures and biochemical characterizations, have provided a deeper understanding of the mechanism and regulation of mitochondrial DNA transcription. In this review, we will discuss recent advances in the structure and mechanism of mitochondrial transcription initiation. We will follow up with recent discoveries and formative findings regarding the regulatory events that control mitochondrial DNA transcription, focusing on those involved in cross-talk between the mitochondria and nucleus.
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U2 - 10.1074/jbc.REV120.011202
DO - 10.1074/jbc.REV120.011202
M3 - Article
C2 - 33127643
AN - SCOPUS:85098250567
SN - 0021-9258
VL - 295
SP - 18406
EP - 18425
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 52
ER -