Structure of a Parallel-Stranded Tetramer of the Oxytricha Telomeric DNA Sequence dT4G4

Goutam Gupta, Angel E. Garcia, Qiu Guo, Min Lu, Neville R. Kallenbach

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

We report here the determination of the solution structure of the single-copy tetramer of the sequence dT4G4, the unit repeat in telomeres of the ciliated protozoan Oxytricha, in the presence of potassium ions. This is accomplished by a combination of sequential assignments and distance determinations from 2D proton NMR with model building, based on conformational analysis of the structure using a full-matrix NOESY simulation and molecular dynamics. Each strand in this tetramer structure has an identical environment and conformation: a parallel-stranded, right-handed helix, with all nucleotides in the C2′-endo, anti configuration. The T flanking the G cluster stacks in a 4-fold symmetrical helical array, while the remaining T's become increasingly flexible and sample multiple stacked configurations.

Original languageEnglish (US)
Pages (from-to)7098-7103
Number of pages6
JournalBiochemistry
Volume32
Issue number28
DOIs
StatePublished - 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Fingerprint

Dive into the research topics of 'Structure of a Parallel-Stranded Tetramer of the Oxytricha Telomeric DNA Sequence dT<sub>4</sub>G<sub>4</sub>'. Together they form a unique fingerprint.

Cite this