Structure of a polycomb response element and in vitro binding of polycomb group complexes containing GAGA factor

Béatrice Horard, Christophe Tatout, Sylvain Poux, Vincenzo Pirrotta

Research output: Contribution to journalArticlepeer-review

166 Scopus citations

Abstract

Polycomb response elements (PREs) are regulatory sites that mediate the silencing of homeotic and other genes. The bxd PRE region from the Drosophila Ultrabithorax gene can be subdivided into subfragments of 100 to 200 bp that retain different degrees of PRE activity in vivo. In vitro, embryonic nuclear extracts form complexes containing Polycomb group (PcG) proteins with these fragments. PcG binding to some fragments is dependent on consensus sequences for the GAGA factor. Other fragments lack GAGA binding sites but can still bind PcG complexes in vitro. We show that the GAGA factor is a component of at least some types of PcG complexes and may participate in the assembly of PcG complexes at PREs.

Original languageEnglish (US)
Pages (from-to)3187-3197
Number of pages11
JournalMolecular and cellular biology
Volume20
Issue number9
DOIs
StatePublished - May 2000

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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