TY - JOUR
T1 - Studies on changes in lipid profiles of the rat gastric mucosa with stress ulcers
AU - Slomiany, Amalia
AU - Slomiany, Bronislaw L.
AU - Horowitz, Martin I.
N1 - Funding Information:
* This research was supported by NIH grant AM1556503 Metabolism and Digestive Diseases. ** Address correspondence to: Martin I. Horowitz, Ph.D., New York Medical College. Department Biochemistry. Basic Science Building, Valhalla. N.Y. 10595, U.S.A.
PY - 1975/3/10
Y1 - 1975/3/10
N2 - Gastric mucosal lipid patterns were studied in normal and in restrained rats which developed mucosal erosions. Organic solvent extraction, silicic acid column, and thin-layer chromatography were employed to separate and gas-liquid chromatography and spectrophotometry to quantitize the individual lipid components. Chemical analysis revealed profound alterations in the mucosal lipid profiles of rats with gastric mucosal erosions. Cholesterol esters were markedly elevated, cholesterol, free fatty acids and phosphatidylethanolamine were markedly lower in the extracts of ulcerated tissue, and the titer of lysophospholipids was about eight times higher than in the controls. Lipid phosphorus was elevated in stressed rats. The amount of neutral glycolipids was similar for both groups, but tetra- and oligohexoside ceramides, which were present in the gastric mucosa of the control rats, were virtually absent from the ulcerated mucosa. In contrast to the alterations in lipid profile mentioned above, the total lipid weight per tissue preparation was very similar for the ulcerated and normal control groups.
AB - Gastric mucosal lipid patterns were studied in normal and in restrained rats which developed mucosal erosions. Organic solvent extraction, silicic acid column, and thin-layer chromatography were employed to separate and gas-liquid chromatography and spectrophotometry to quantitize the individual lipid components. Chemical analysis revealed profound alterations in the mucosal lipid profiles of rats with gastric mucosal erosions. Cholesterol esters were markedly elevated, cholesterol, free fatty acids and phosphatidylethanolamine were markedly lower in the extracts of ulcerated tissue, and the titer of lysophospholipids was about eight times higher than in the controls. Lipid phosphorus was elevated in stressed rats. The amount of neutral glycolipids was similar for both groups, but tetra- and oligohexoside ceramides, which were present in the gastric mucosa of the control rats, were virtually absent from the ulcerated mucosa. In contrast to the alterations in lipid profile mentioned above, the total lipid weight per tissue preparation was very similar for the ulcerated and normal control groups.
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U2 - 10.1016/0009-8981(75)90032-7
DO - 10.1016/0009-8981(75)90032-7
M3 - Article
C2 - 164307
AN - SCOPUS:0016606489
SN - 0009-8981
VL - 59
SP - 215
EP - 226
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -