Study of ganglioside patterns with two-dimensional thin-layer chromatography and radioautography; Detection of new fucogangliosides and other minor species in rabbit brain

Robert Ledeen, James E. Haley, Joseph A. Skrivanek

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Brain gangliosides are shown to be highly complex mixtures of major and minor species through the use of two-dimensional thin-layer chromatography combined with radioautography. When applied to 14C-labeled gangliosides, radioautography could be employed as a sensitive detection procedure capable of revealing minor species that were not visualized with a conventional spray. The brain ganglioside pattern of neonatal rabbits was studied in this manner following intracranial injection of 14C-labeled N-acetylmannosamine and fucose. Numerous minor components representing previously uncharacterized gangliosides were detected, including several new fucose-containing gangliosides.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalAnalytical Biochemistry
Volume112
Issue number1
DOIs
StatePublished - Mar 15 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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