Brain gangliosides are shown to be highly complex mixtures of major and minor species through the use of two-dimensional thin-layer chromatography combined with radioautography. When applied to 14C-labeled gangliosides, radioautography could be employed as a sensitive detection procedure capable of revealing minor species that were not visualized with a conventional spray. The brain ganglioside pattern of neonatal rabbits was studied in this manner following intracranial injection of 14C-labeled N-acetylmannosamine and fucose. Numerous minor components representing previously uncharacterized gangliosides were detected, including several new fucose-containing gangliosides.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology