Study on the usefulness of hypertonic culture media

D. B. Louria, T. Kaminski, R. Kapila, F. Tecson, L. Smith

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Specimens from 300 patients were studied using five to nine aerobic and anaerobic culture media, including five that were hypertonic. Groups studied included fever of unknown origin, suspected endocarditis, endocarditis during therapy, bacteremia during therapy, abscess and cellulitis, presumed infectious arthritis, renal transplantation during rejection, collagen disease, sarcoidosis, lymphoma, and colitis. Isolates in hypertonic media were reverted to parent form by agar passage. In only 5% of these selected cases were organisms found in hypertonic, but not conventional, media that appeared on the basis of repeated isolation and/or serological studies to come from the patient. Nine of the 16 appeared to be of major significance. The two groups in which use of highly enriched, hypertonic media seemed most helpful were suspected endocarditis and undefined meningitis with negative cultures using standard media. The most effective of the hypertonic media used was 0.3 M sucrose in brain heart infusion with 20% horse serum. In most instances, the organism grew only in the hypertonic sucrose, and in most cases it appeared in conventional rather than aberrant form. Hypertonic media, especially 0.3 M sucrose, are of substantial help in a small number of carefully selected cases.

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalJournal of clinical microbiology
Volume4
Issue number3
StatePublished - 1976

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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