subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster

Kelly L. Giunta, Janet K. Jang, Elizabeth A. Manheim, Gayathri Subramanian, Kim McKim

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The female meiotic spindle lacks a centrosome or microtubule-organizing center in many organisms. During cell division, these spindles are organized by the chromosomes and microtubule-associated proteins. Previous studies in Drosophila melanogaster implicated at least one kinesin motor protein, NCD, in tapering the microtubules into a bipolar spindle. We have identified a second Drosophila kinesin-like protein, SUB, that is required for meiotic spindle function. At meiosis I in males and females, sub mutations affect only the segregation of homologous chromosomes. In female meiosis, sub mutations have a similar phenotype to ncd; even though chromosomes are joined by chiasmata they fail to segregate at meiosis I. Cytological analyses have revealed that sub is required for bipolar spindle formation. In sub mutations, we observed spindles that were unipolar, multipolar, or frayed with no defined poles. On the basis of these phenotypes and the observation that sub mutations genetically interact with ncd, we propose that SUB is one member of a group of microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosomes, sub is also required for the early embryonic divisions but is otherwise dispensable for most mitotic divisions.

Original languageEnglish (US)
Pages (from-to)1489-1501
Number of pages13
JournalGenetics
Volume160
Issue number4
StatePublished - May 9 2002

Fingerprint

Spindle Poles
Kinesin
Spindle Apparatus
Drosophila melanogaster
Meiosis
Centrosome
Mutation
Microtubule-Associated Proteins
Proteins
Chromosomes
Microtubule-Organizing Center
Phenotype
Chromosome Segregation
Microtubules
Cell Division
Drosophila

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Giunta, K. L., Jang, J. K., Manheim, E. A., Subramanian, G., & McKim, K. (2002). subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster. Genetics, 160(4), 1489-1501.
Giunta, Kelly L. ; Jang, Janet K. ; Manheim, Elizabeth A. ; Subramanian, Gayathri ; McKim, Kim. / subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster. In: Genetics. 2002 ; Vol. 160, No. 4. pp. 1489-1501.
@article{fd78c44d7ec04e74b1b95e1e6ed3fdd3,
title = "subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster",
abstract = "The female meiotic spindle lacks a centrosome or microtubule-organizing center in many organisms. During cell division, these spindles are organized by the chromosomes and microtubule-associated proteins. Previous studies in Drosophila melanogaster implicated at least one kinesin motor protein, NCD, in tapering the microtubules into a bipolar spindle. We have identified a second Drosophila kinesin-like protein, SUB, that is required for meiotic spindle function. At meiosis I in males and females, sub mutations affect only the segregation of homologous chromosomes. In female meiosis, sub mutations have a similar phenotype to ncd; even though chromosomes are joined by chiasmata they fail to segregate at meiosis I. Cytological analyses have revealed that sub is required for bipolar spindle formation. In sub mutations, we observed spindles that were unipolar, multipolar, or frayed with no defined poles. On the basis of these phenotypes and the observation that sub mutations genetically interact with ncd, we propose that SUB is one member of a group of microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosomes, sub is also required for the early embryonic divisions but is otherwise dispensable for most mitotic divisions.",
author = "Giunta, {Kelly L.} and Jang, {Janet K.} and Manheim, {Elizabeth A.} and Gayathri Subramanian and Kim McKim",
year = "2002",
month = "5",
day = "9",
language = "English (US)",
volume = "160",
pages = "1489--1501",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",

}

Giunta, KL, Jang, JK, Manheim, EA, Subramanian, G & McKim, K 2002, 'subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster', Genetics, vol. 160, no. 4, pp. 1489-1501.

subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster. / Giunta, Kelly L.; Jang, Janet K.; Manheim, Elizabeth A.; Subramanian, Gayathri; McKim, Kim.

In: Genetics, Vol. 160, No. 4, 09.05.2002, p. 1489-1501.

Research output: Contribution to journalArticle

TY - JOUR

T1 - subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster

AU - Giunta, Kelly L.

AU - Jang, Janet K.

AU - Manheim, Elizabeth A.

AU - Subramanian, Gayathri

AU - McKim, Kim

PY - 2002/5/9

Y1 - 2002/5/9

N2 - The female meiotic spindle lacks a centrosome or microtubule-organizing center in many organisms. During cell division, these spindles are organized by the chromosomes and microtubule-associated proteins. Previous studies in Drosophila melanogaster implicated at least one kinesin motor protein, NCD, in tapering the microtubules into a bipolar spindle. We have identified a second Drosophila kinesin-like protein, SUB, that is required for meiotic spindle function. At meiosis I in males and females, sub mutations affect only the segregation of homologous chromosomes. In female meiosis, sub mutations have a similar phenotype to ncd; even though chromosomes are joined by chiasmata they fail to segregate at meiosis I. Cytological analyses have revealed that sub is required for bipolar spindle formation. In sub mutations, we observed spindles that were unipolar, multipolar, or frayed with no defined poles. On the basis of these phenotypes and the observation that sub mutations genetically interact with ncd, we propose that SUB is one member of a group of microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosomes, sub is also required for the early embryonic divisions but is otherwise dispensable for most mitotic divisions.

AB - The female meiotic spindle lacks a centrosome or microtubule-organizing center in many organisms. During cell division, these spindles are organized by the chromosomes and microtubule-associated proteins. Previous studies in Drosophila melanogaster implicated at least one kinesin motor protein, NCD, in tapering the microtubules into a bipolar spindle. We have identified a second Drosophila kinesin-like protein, SUB, that is required for meiotic spindle function. At meiosis I in males and females, sub mutations affect only the segregation of homologous chromosomes. In female meiosis, sub mutations have a similar phenotype to ncd; even though chromosomes are joined by chiasmata they fail to segregate at meiosis I. Cytological analyses have revealed that sub is required for bipolar spindle formation. In sub mutations, we observed spindles that were unipolar, multipolar, or frayed with no defined poles. On the basis of these phenotypes and the observation that sub mutations genetically interact with ncd, we propose that SUB is one member of a group of microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosomes, sub is also required for the early embryonic divisions but is otherwise dispensable for most mitotic divisions.

UR - http://www.scopus.com/inward/record.url?scp=0036238557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036238557&partnerID=8YFLogxK

M3 - Article

C2 - 11973304

AN - SCOPUS:0036238557

VL - 160

SP - 1489

EP - 1501

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -