Substance P gene expression is regulated by interleukin‐1 in cultured sympathetic ganglia

R. P. Hart, A. M. Shadiack, G. M. Jonakait

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

We have investigated the effects of interleukin‐1β (IL‐1β) on the induction of substance P (SP) in cultured sympathetic ganglia. Northern blot analysis reveals that SP increases are secondary to an increase in mRNA coding for the preprotachykinin (PPT) precursor of SP. Nuclear transcription assays detect an early increase in PPT‐specific nascent transcripts, suggesting that the ultimate effect of IL‐1 is on transcription itself. Depolarizing agents, interferon‐γ glucocorticoid hormones, and prostaglandin synthesis inhibitors all diminish the induction of SP and PPT mRNA by IL‐1. Since SP has stimulatory effects on the immune system, the IL‐1–induced increase in ganglionic SP may be one means by which the nervous and immune systems interact during an acute response to ganglionic injury.

Original languageEnglish (US)
Pages (from-to)282-291
Number of pages10
JournalJournal of Neuroscience Research
Volume29
Issue number3
DOIs
StatePublished - Jul 1991

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Keywords

  • glucocorticoid hormones
  • interferon‐γ
  • neuronal injury
  • preprotachykinin mRNA
  • superior cervical ganglion
  • sympathetic nervous system

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