[125I]Orphanin FQ/nociceptin binding in Raji cells

Judith S.H. Hom, Ira Goldberg, John Mathis, Ying Xian Pan, Andrew I. Brooks, Jennifer Ryan-Moro, David A. Scheinberg, Gavril W. Pasternak

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Western blots using an antibody which recognizes the orphanin FQ/ nociceptin (OFQ/N) receptor reveals a band at approximately 69 kD in several cell lines, including the Raji human B cell lymphoma cell line. RT-PCR confirms the presence of this receptor in the Raji cells. Binding studies revealed a high affinity [125I][Tyr14]OFQ/N site in the Raji cells. The affinity of [125I] [Tyr14]OFQ/N in the Raji cells (K(D) 68.4 pM) was similar to that in the transfected receptor (K(D) 36.7 pM). Its selectivity profile also was quite similar. OFQ/N competed binding quite potently (K(i) 65 pM), as did [Tyr14]OFQ/N (K(i) 33 pM). Traditional opioids displayed no appreciable affinity for the binding at any concentration examined, with the exception of naloxone benzoylhydrazone, which had only a very modest affinity. The receptors in the Raji cells were functionally active. OFQ/N inhibited forskolin-stimulated cyclase by 72% with an IC50 value of approximately 1 nM.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
Issue number3
StatePublished - 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience


  • Immune cells
  • KOR-3
  • Nociceptin
  • ORL1
  • Orphanin FQ


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