14C-cobalamin absorption from endogenously labeled chicken eggs assessed in humans using accelerator mass spectrometry

Marjorie G. Garrod, Heidi A. Rossow, Christopher C. Calvert, Joshua W. Miller, Ralph Green, Bruce A. Buchholz, Lindsay H. Allen

Research output: Contribution to journalArticle

Abstract

Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 µg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 µg only half that from 1.4 µg. The total amount of B12 absorbed was limited to 0.5-0.8 µg (mean 0.55 ± 0.19 µg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 µg/day).

Original languageEnglish (US)
Article number2148
JournalNutrients
Volume11
Issue number9
DOIs
StatePublished - Sep 2019

Fingerprint

chicken eggs
vitamin B12
Vitamin B 12
Eggs
Biological Availability
bioavailability
Chickens
Mass Spectrometry
mass spectrometry
Vitamins
vitamins
Food
dosage
Radioactivity
volunteers
Ovum
hens
Volunteers
urine
Eating

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

Keywords

  • Accelerator mass spectrometry
  • Bioavailability
  • Cobalamin
  • Eggs
  • Endogenous label
  • Human
  • Vitamin B12

Cite this

Garrod, Marjorie G. ; Rossow, Heidi A. ; Calvert, Christopher C. ; Miller, Joshua W. ; Green, Ralph ; Buchholz, Bruce A. ; Allen, Lindsay H. / 14C-cobalamin absorption from endogenously labeled chicken eggs assessed in humans using accelerator mass spectrometry. In: Nutrients. 2019 ; Vol. 11, No. 9.
@article{b37dc05bd4d04e6bb277c5786e42fefc,
title = "14C-cobalamin absorption from endogenously labeled chicken eggs assessed in humans using accelerator mass spectrometry",
abstract = "Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 µg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7{\%} (mean 30.2 ± 16.4{\%}). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 µg only half that from 1.4 µg. The total amount of B12 absorbed was limited to 0.5-0.8 µg (mean 0.55 ± 0.19 µg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20{\%} of the average adult daily requirement (RDA for adults = 2.4 µg/day).",
keywords = "Accelerator mass spectrometry, Bioavailability, Cobalamin, Eggs, Endogenous label, Human, Vitamin B12",
author = "Garrod, {Marjorie G.} and Rossow, {Heidi A.} and Calvert, {Christopher C.} and Miller, {Joshua W.} and Ralph Green and Buchholz, {Bruce A.} and Allen, {Lindsay H.}",
year = "2019",
month = "9",
doi = "10.3390/nu11092148",
language = "English (US)",
volume = "11",
journal = "Nutrients",
issn = "2072-6643",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

14C-cobalamin absorption from endogenously labeled chicken eggs assessed in humans using accelerator mass spectrometry. / Garrod, Marjorie G.; Rossow, Heidi A.; Calvert, Christopher C.; Miller, Joshua W.; Green, Ralph; Buchholz, Bruce A.; Allen, Lindsay H.

In: Nutrients, Vol. 11, No. 9, 2148, 09.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 14C-cobalamin absorption from endogenously labeled chicken eggs assessed in humans using accelerator mass spectrometry

AU - Garrod, Marjorie G.

AU - Rossow, Heidi A.

AU - Calvert, Christopher C.

AU - Miller, Joshua W.

AU - Green, Ralph

AU - Buchholz, Bruce A.

AU - Allen, Lindsay H.

PY - 2019/9

Y1 - 2019/9

N2 - Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 µg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 µg only half that from 1.4 µg. The total amount of B12 absorbed was limited to 0.5-0.8 µg (mean 0.55 ± 0.19 µg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 µg/day).

AB - Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 µg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 µg only half that from 1.4 µg. The total amount of B12 absorbed was limited to 0.5-0.8 µg (mean 0.55 ± 0.19 µg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 µg/day).

KW - Accelerator mass spectrometry

KW - Bioavailability

KW - Cobalamin

KW - Eggs

KW - Endogenous label

KW - Human

KW - Vitamin B12

UR - http://www.scopus.com/inward/record.url?scp=85071971626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071971626&partnerID=8YFLogxK

U2 - 10.3390/nu11092148

DO - 10.3390/nu11092148

M3 - Article

C2 - 31500393

AN - SCOPUS:85071971626

VL - 11

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 9

M1 - 2148

ER -