Suppression of arachidonic acid metabolism and nitric oxide formation by kudzu isoflavones in murine macrophages

Mira Jun, Jungil Hong, Woo Sik Jeong, Chi Tang Ho

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Inhibitory effect of kudzu isoflavones on arachidonic acid metabolism and nitric oxide (NO) production in lipopolysaccharide activated RAW 264.7 macrophages were investigated. Isoflavone aglycones, such as daidzein, genistein, biochanin A, and formononetin significantly suppressed arachidonic acid release (50 μM). Biochanin A, which displayed the most active inhibition on arachidonic acid release in HT-29 human colon cancer cells, exhibited its most potent suppression in RAW 264.7 cell (by 86%) without showing cytotoxicity. However, isoflavone glucosides, puerarin and daidzin, showed lower inhibitory activities on the release of arachidonic acid and its metabolites. In NO formation, biochanin A showed marked inhibition, by 62% (50 μM), followed by genistein, daidzein, formononetin, and daidzin, 56, 39, 33, and 8%, respectively. 5,7-Dihydroxyl group in the A-ring of isoflavones could be a key functional group responsible for the strong inhibitory activity of biochanin A and genistein on NO production. These activities may contribute to the antiinflammatory and anticarcinogenic properties of kudzu isoflavones.

Original languageEnglish (US)
Pages (from-to)1154-1159
Number of pages6
JournalMolecular Nutrition and Food Research
Volume49
Issue number12
DOIs
StatePublished - Dec 2005

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

Keywords

  • Arachidonic acid
  • Isoflavones
  • Kudzu
  • Macrophage
  • Nitric oxide

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