Suppression of endothelin-converting enzyme-1 during buccal mucosal ulcer healing: Effect of chronic alcohol ingestion

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Among the factors affecting the efficiency of soft oral tissue healing is endothelin-1 (ET-1), a potent vasoactive peptide produced from a biologically inactive big ET-1 by the action of endothelin-converting enzyme-1 (ECE-1). We investigated the expression of ECE-1 during buccal mucosal ulcer healing in rats maintained for 5 weeks on alcohol containing or control diet. The mucosal activity of ECE-1, characterized by sensitivity to phosphoramidon, was associated with microsomal fraction and showed an elevated (3.1-fold) level in the alcohol diet group. Moreover, the ulcer onset in the alcohol group was reflected in a 3.1 greater expression of ECE-1 activity, and was accompanied by a 1.4-fold greater increase in TNF-α and a 2.5-fold greater enhancement in epithelial cell apoptosis. While in both groups the ulcer healing was associated with a decrease in buccal mucosal expression of ECE-1, as well as a decline in TNF-α and apoptosis, the changes were significantly slower in the alcohol diet group and manifested by a 40% delay in healing. Thus, chronic alcohol ingestion leads to up-regulation of ECE-1 expression, induction of TNF-α, and triggering apoptotic events that delay the mucosal repair. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)318-322
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume271
Issue number2
DOIs
StatePublished - May 10 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Alcoholism
  • Apoptosis
  • Buccal mucosa
  • ECE-1
  • TNF-α
  • Ulcer healing

Fingerprint Dive into the research topics of 'Suppression of endothelin-converting enzyme-1 during buccal mucosal ulcer healing: Effect of chronic alcohol ingestion'. Together they form a unique fingerprint.

Cite this