Abstract
To evaluate the contributions of the pre- versus postsynaptic expression of NCAM in regulation of synaptic efficacy, we cultured dissociated hippocampal cells from NCAM-deficient and wild-type mice in homo- and heterogenotypic combinations. Double recordings from synaptically coupled neurons maintained in heterogenotypic cocultures showed that synaptic strength of excitatory but not inhibitory synapses depended on expression of NCAM post- but not presynaptically. This correlated with higher levels of potentiation and synaptic coverage of NCAM-expressing neurons compared to NCAM-deficient neurons in heterogenotypic cocultures. Synaptic density was the same in homogenotypic cultures of NCAM-deficient and wild-type neurons as well as in heterogenotypic cocultures in which glutamate receptors were blocked. These observations indicate that the relative levels of postsynaptic NCAM expression control synaptic strength in an activity-dependent manner by regulating the number of synapses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 207-217 |
| Number of pages | 11 |
| Journal | Neuron |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2000 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Synaptic strength as a function of post- versus presynaptic expression of the neural cell adhesion molecule NCAM. / Dityatev, Alexander; Dityateva, Galina; Camartin, Melitta.
In: Neuron, Vol. 26, No. 1, 01.01.2000, p. 207-217.Research output: Contribution to journal › Article
TY - JOUR
T1 - Synaptic strength as a function of post- versus presynaptic expression of the neural cell adhesion molecule NCAM
AU - Dityatev, Alexander
AU - Dityateva, Galina
AU - Camartin, Melitta
PY - 2000/1/1
Y1 - 2000/1/1
N2 - To evaluate the contributions of the pre- versus postsynaptic expression of NCAM in regulation of synaptic efficacy, we cultured dissociated hippocampal cells from NCAM-deficient and wild-type mice in homo- and heterogenotypic combinations. Double recordings from synaptically coupled neurons maintained in heterogenotypic cocultures showed that synaptic strength of excitatory but not inhibitory synapses depended on expression of NCAM post- but not presynaptically. This correlated with higher levels of potentiation and synaptic coverage of NCAM-expressing neurons compared to NCAM-deficient neurons in heterogenotypic cocultures. Synaptic density was the same in homogenotypic cultures of NCAM-deficient and wild-type neurons as well as in heterogenotypic cocultures in which glutamate receptors were blocked. These observations indicate that the relative levels of postsynaptic NCAM expression control synaptic strength in an activity-dependent manner by regulating the number of synapses.
AB - To evaluate the contributions of the pre- versus postsynaptic expression of NCAM in regulation of synaptic efficacy, we cultured dissociated hippocampal cells from NCAM-deficient and wild-type mice in homo- and heterogenotypic combinations. Double recordings from synaptically coupled neurons maintained in heterogenotypic cocultures showed that synaptic strength of excitatory but not inhibitory synapses depended on expression of NCAM post- but not presynaptically. This correlated with higher levels of potentiation and synaptic coverage of NCAM-expressing neurons compared to NCAM-deficient neurons in heterogenotypic cocultures. Synaptic density was the same in homogenotypic cultures of NCAM-deficient and wild-type neurons as well as in heterogenotypic cocultures in which glutamate receptors were blocked. These observations indicate that the relative levels of postsynaptic NCAM expression control synaptic strength in an activity-dependent manner by regulating the number of synapses.
UR - http://www.scopus.com/inward/record.url?scp=0033679834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033679834&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)81151-4
DO - 10.1016/S0896-6273(00)81151-4
M3 - Article
C2 - 10798405
AN - SCOPUS:0033679834
VL - 26
SP - 207
EP - 217
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -