Synthesis of compositionally unique DNA by terminal deoxynucleotidyl transferase

Mitali Basu, Mahabaleshwar V. Hegde, Mukund J. Modak

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Studies on the composition and characterization of DNA product(s) synthesized by calf thymus terminal deoxynucleotidyl transferase were performed using homopolymeric single-stranded, calf thymus double-stranded, and native DNA resident in calf thymus chromatin preparations as priming DNA species. Synthesis was carried out using equimolar concentrations of all four deoxynucleoside triphosphates as substrates and Mg2+ or Mn2+ as an effective divalent cation. Irrespective of the nature of the priming DNA or the divalent cation, the DNA product contained 60-70% dGMP residues, 10-15% each of the two pyrimidine residues, and 5-10% dAMP residues. The product synthesized using chromatin DNA as initiator was predominantly single-stranded and its synthesis was resistant to actinomycin D. The predilection of terminal deoxynucleotidyl transfease to synthesize dGMP-rich products on natural or homopolymeric DNA primers suggests that such products may represent biologically important recognition signal sequences.

Original languageEnglish (US)
Pages (from-to)1105-1112
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume111
Issue number3
DOIs
StatePublished - Mar 29 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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