Synthesis of DNA complementary to AMV RNA using E. coli polymerase I

Mukund J. Modak, Stuart L. Marcus, Liebe F. Cavalieri

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

E. coli DNA polymerase I has been used to copy avian myeloblastosis virus RNA both in the presence and absence of the primer oligo-dT10. Heteropolymer synthesis is obtained in both cases. The product DNA obtained using the primer has been shown to be complementary to the RNA template. Substantial homopolymer synthesis (poly dT and poly (dA)·(dT)) occurs in the presence and very little in the absence of the oligo dT10 primer.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume56
Issue number1
DOIs
StatePublished - Jan 7 1974
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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