E. coli DNA polymerase I has been used to copy avian myeloblastosis virus RNA both in the presence and absence of the primer oligo-dT10. Heteropolymer synthesis is obtained in both cases. The product DNA obtained using the primer has been shown to be complementary to the RNA template. Substantial homopolymer synthesis (poly dT and poly (dA)·(dT)) occurs in the presence and very little in the absence of the oligo dT10 primer.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 7 1974|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology