Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro

D. M. Lawrence, B. I. Hillman

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Blueberry scorch carlavirus (BBScV) is a filamentous virus with a polyadenylated, positive-sense RNA genome. A near full-length cDNA clone of BBScV was constructed by assembly of clones from a cDNA library. To generate a full-length cDNA clone, the 5' terminus was mutagenized by PCR to introduce nucleotides present in the wild-type virus and not in the near full-length clone, and then fused directly to the T7 bacteriophage RNA polymerase promoter at the 5' terminus. Capped and uncapped BBScV transcripts were synthesized in vitro from the full-length cDNA clone. Capped transcripts were infectious, producing systemic symptoms identical to those caused by the wild-type virus following inoculation onto Chenopodium quinoa leaves. Uncapped transcripts were substantially less infectious than capped transcripts. This represents the first report of infectious transcripts for a member of the carlavirus group.

Original languageEnglish (US)
Pages (from-to)2509-2512
Number of pages4
JournalJournal of General Virology
Volume75
Issue number9
DOIs
StatePublished - 1994

All Science Journal Classification (ASJC) codes

  • Virology

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