Synthesis of sterigmatocystin on a chemically defined medium by species of Aspergillus and Chaetomium

S. E. Barnes, T. P. Dola, Joan Bennett, D. Bhatnagar

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Sterigmatocystin (ST) is a secondary metabolite and a principal mycotoxin known to be produced by over 30 species of filamentous fungi. It is also one of the late intermediates in aflatoxin biosynthesis. We have tested the ability of 7 species of Aspergillus, including 4 strains of A. versicolor, one species of Bipolaris, and two species of Chaetomium, to produce ST on a sucrose-salts-phenylalanine defined medium as well as on three complex substrates. Highest ST production in our survey was by a strain of A. versicolor grown on wheat, whereas, the highest ST production on defined medium was by C. cellulolyticum. To our knowledge, this is the first report of ST production by C. cellulolyticum on any substrate. In precursor feeding studies, resting cultures of wild type A. nidulans and A. versicolor were unable to biotransform O-methylsterigmatocystin (OMST), the last known intermediate in aflatoxin biosynthesis. These results suggest that ST is the end product of polyketide metabolism in the strains tested.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalMycopathologia
Volume125
Issue number3
DOIs
StatePublished - Mar 1 1994
Externally publishedYes

Fingerprint

Sterigmatocystin
Chaetomium
sterigmatocystin
Aspergillus
synthesis
Aflatoxins
aflatoxins
biosynthesis
Aspergillus versicolor
Bipolaris
Polyketides
polyketides
Mycotoxins
Phenylalanine
phenylalanine
mycotoxins
secondary metabolites
Triticum
Sucrose
Fungi

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Barnes, S. E. ; Dola, T. P. ; Bennett, Joan ; Bhatnagar, D. / Synthesis of sterigmatocystin on a chemically defined medium by species of Aspergillus and Chaetomium. In: Mycopathologia. 1994 ; Vol. 125, No. 3. pp. 173-178.
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Synthesis of sterigmatocystin on a chemically defined medium by species of Aspergillus and Chaetomium. / Barnes, S. E.; Dola, T. P.; Bennett, Joan; Bhatnagar, D.

In: Mycopathologia, Vol. 125, No. 3, 01.03.1994, p. 173-178.

Research output: Contribution to journalArticle

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