System for cleavable Fc fusion proteins using tobacco etch virus (TEV) protease

J. Haspel, C. Blanco, J. Jacob, Martin Grumet

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We describe a novel Fc fusion protein system that can be cleaved by tobacco etch virus (TEV) protease. This system is desirable because it takes advantage of the high specificity of TEV protease and its activity at 4°C. We produced two TEV-Fc fusion proteins that contain the first three Ig domains and all six Ig domains of the cell adhesion molecule L1. Both proteins were efficiently cleaved by TEV protease at 4°C. Functional analysis of the cleavage products in neurite outgrowth assays showed they had similar activities to their parental Fc fusion proteins. Therefore, TEV-Fc fusion proteins may increase the utility and flexibility of the Fc fusion protein system.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalBioTechniques
Volume30
Issue number1
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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