Systematic Reverse Genetic Screening of T-DNA Tagged Genes in Rice for Functional Genomic Analyses: MADS-box Genes as a Test Case

Shinyoung Lee, Joonyul Kim, Jun Seock Son, Jongmin Nam, Dong Hoon Jeong, Keunsub Lee, Seonghoe Jang, Jihye Yoo, Jinwon Lee, Dong Yeon Lee, Hong Gyu Kang, Gynheung An

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to genean@postech.ac.kr.

Original languageEnglish (US)
Pages (from-to)1403-1411
Number of pages9
JournalPlant and Cell Physiology
Volume44
Issue number12
DOIs
StatePublished - Dec 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Keywords

  • Insertional mutagenesis
  • MADS-box genes
  • Pool screening
  • Rice
  • T-DNA

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