T cell receptor DJ but not VDJ rearrangement within a recombination substrate introduced into a pre-b cell line

Pierre Ferrler, Lori R. Covey, Helkyung Suh, Aster Wlnoto, Leroy Hood, Frederick W. Alt

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

To elucidate mechanisms that regulate ordered and tissue-specific assembly of Ig and TCR variable region gene segments, we have Introduced a recombination substrate comprised of germline TCRβ V, D, and J gene segments Into an Abelson murine leukemia virus-transformed pre-B cell line that actively rearranges endogenous Ig H chain variable region gene segments but does not rearrange endogenous light chain or TCR variable region gene segments. We find that these cells efficiently Join Dβ segments to Jβ segments within the mlnl-locus, but that they do not make any detectable site-specific rearrangements of the Introduced Vβ segment even though It Is closely linked In the same construct to the Dβ. These findings suggest that factors necessary for Vβ to (Dβ)Jβ Joining may be absent In these pre-B cells and also Imply that the order In which TCR Vβ Dβ, and Jβ segments are rearranged can be Influenced by factors other than the 12/23 recombination rule. Furthermore, In agreement with the an accessibility model of Vβ recombinase control, the Dβ region of the construct was found to be relatively more sensitive to DNAase I digestion In Isolated nuclei when compared to the unrearranged Vβ region.

Original languageEnglish (US)
Pages (from-to)66-74
Number of pages9
JournalInternational Immunology
Volume1
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • DNAase I sensitivity
  • Gene transfer
  • VDJ recombinase

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