Mechanisms that lead to traumatic brain injury due to blast exposure (bTBI) are not well understood. Complexities of cellular responses involved have made identification of these mechanisms challenging. In-vitro studies of blast induced traumatic brain injury are possible with employment of shock tubes which closely mimic the loading conditions of blasts in a laboratory setting. A novel pneumatic, two-chamber shock tube has been developed to generate a span of mild to severe pressure waveforms seen in TBI literature [1-2]. Unlike other shock tubes utilized in TBI research, it is small, portable, controllable and most importantly safe to operate. The ultimate goal is to use this device to develop a blast induced traumatic brain injury animal model that can be used to explain the injury mechanism(s) and threshold levels of brain injury after blast exposure.