Targeted cell detection based on microchannel gating

Mehdi Javanmard, Amir Ali H. Talasaz, Mohsen Nemat-Gorgani, Fabian Pease, Mostafa Ronaghi, Ronald W. Davis

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Currently, microbiological techniques such as culture enrichment and various plating techniques are used for detection of pathogens. These expensive and time consuming methods can take several days. Described below is the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a microchannel, which can be used to detect single bacterial cells electrically (label-free format) in real time. As a proof of principle, we have successfully demonstrated real-time detection of target yeast cells by measuring instantaneous changes in ionic impedance. We have also demonstrated the selectivity of our sensors in responding to target cells while remaining irresponsive to nontarget cells. Using this technique, it can be possible to multiplex an array of these sensors onto a chip and probe a complex mixture for various types of bacterial cells.

Original languageEnglish (US)
Article number044103
JournalBiomicrofluidics
Volume1
Issue number4
DOIs
StatePublished - Jan 1 2007

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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