Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids

M. Sugita; Z. Svab; P. Maliga; M. Sugiura

doi:10.1007/s004380050619

Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids

M. Sugita, Z. Svab, P. Maliga, M. Sugiura

Waksman Institute of Microbiology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The small plastid RNA (spRNA) which includes a segment that is complementary to the pre-16S rRNA has been suggested to facilitate maturation of pre-16S rRNA in tobacco. To investigate the function of spRNA, the gene encoding it (sprA) was removed from the plastid genome using targeted gene deletion. We report here that deletion of sprA does not significantly affect pre-16S rRNA maturation, nor does it cause any obvious phenotype. Although the spRNA still may be involved in rRNA maturation, it is non-essential under normal growth conditions.

Original language	English (US)
Pages (from-to)	23-27
Number of pages	5
Journal	Molecular and General Genetics
Volume	257
Issue number	1
DOIs	https://doi.org/10.1007/s004380050619
State	Published - 1997

All Science Journal Classification (ASJC) codes

Genetics

Keywords

Maturation of pre-16S rRNA
Targeted gene deletion
Tobacco
spRNA
sprA gene

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10.1007/s004380050619

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title = "Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids",

abstract = "The small plastid RNA (spRNA) which includes a segment that is complementary to the pre-16S rRNA has been suggested to facilitate maturation of pre-16S rRNA in tobacco. To investigate the function of spRNA, the gene encoding it (sprA) was removed from the plastid genome using targeted gene deletion. We report here that deletion of sprA does not significantly affect pre-16S rRNA maturation, nor does it cause any obvious phenotype. Although the spRNA still may be involved in rRNA maturation, it is non-essential under normal growth conditions.",

keywords = "Maturation of pre-16S rRNA, Targeted gene deletion, Tobacco, spRNA, sprA gene",

author = "M. Sugita and Z. Svab and P. Maliga and M. Sugiura",

note = "Funding Information: Acknowledgments We thank T. Hirose for useful discussions, J. Y. Suzuki for critical reading of this manuscript, and C. Sugita for construction of plasmids. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, Sports and Culture and by a program grant (Pioneering Research Projects in Biotechnology) from the Ministry of Agriculture, Forestry and Fisheries, Japan (M.S.) and the National Science Foundation, USA (to P.M.).",

year = "1997",

doi = "10.1007/s004380050619",

language = "English (US)",

volume = "257",

pages = "23--27",

journal = "Molecular and General Genetics",

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T1 - Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids

AU - Sugita, M.

AU - Svab, Z.

AU - Maliga, P.

AU - Sugiura, M.

N1 - Funding Information: Acknowledgments We thank T. Hirose for useful discussions, J. Y. Suzuki for critical reading of this manuscript, and C. Sugita for construction of plasmids. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, Sports and Culture and by a program grant (Pioneering Research Projects in Biotechnology) from the Ministry of Agriculture, Forestry and Fisheries, Japan (M.S.) and the National Science Foundation, USA (to P.M.).

PY - 1997

Y1 - 1997

N2 - The small plastid RNA (spRNA) which includes a segment that is complementary to the pre-16S rRNA has been suggested to facilitate maturation of pre-16S rRNA in tobacco. To investigate the function of spRNA, the gene encoding it (sprA) was removed from the plastid genome using targeted gene deletion. We report here that deletion of sprA does not significantly affect pre-16S rRNA maturation, nor does it cause any obvious phenotype. Although the spRNA still may be involved in rRNA maturation, it is non-essential under normal growth conditions.

AB - The small plastid RNA (spRNA) which includes a segment that is complementary to the pre-16S rRNA has been suggested to facilitate maturation of pre-16S rRNA in tobacco. To investigate the function of spRNA, the gene encoding it (sprA) was removed from the plastid genome using targeted gene deletion. We report here that deletion of sprA does not significantly affect pre-16S rRNA maturation, nor does it cause any obvious phenotype. Although the spRNA still may be involved in rRNA maturation, it is non-essential under normal growth conditions.

KW - Maturation of pre-16S rRNA

KW - Targeted gene deletion

KW - Tobacco

KW - spRNA

KW - sprA gene

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DO - 10.1007/s004380050619

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Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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