Targeted gene mutation of E2F1 evokes age-dependent synaptic disruption and behavioral deficits

Jenhao H. Ting, David R. Marks, Stephanie S. Schleidt, Joanna N. Wu, Jacob W. Zyskind, Kathryn A. Lindl, Julie A. Blendy, R. Christopher Pierce, Kelly L. Jordan-Sciutto

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Aberrant expression and activation of the cell cycle protein E2F1 in neurons has been implicated in many neurodegenerative diseases. As a transcription factor regulating G1 to S phase progression in proliferative cells, E2F1 is often up-regulated and activated in models of neuronal death. However, despite its well-studied functions in neuronal death, little is known regarding the role of E2F1 in the mature brain. In this study, we used a combined approach to study the effect of E2F1 gene disruption on mouse behavior and brain biochemistry. We identified significant age-dependent olfactory and memory-related deficits in E2f1 mutant mice. In addition, we found that E2F1 exhibits punctated staining and localizes closely to the synapse. Furthermore, we found a mirroring age-dependent loss of post-synaptic protein-95 in the hippocampus and olfactory bulb as well as a global loss of several other synaptic proteins. Coincidently, E2F1 expression is significantly elevated at the ages, in which behavioral and synaptic perturbations were observed. Finally, we show that deficits in adult neurogenesis persist late in aged E2f1 mutant mice which may partially contribute to the behavior phenotypes. Taken together, our data suggest that the disruption of E2F1 function leads to specific age-dependent behavioral deficits and synaptic perturbations.

Original languageEnglish (US)
Pages (from-to)850-863
Number of pages14
JournalJournal of neurochemistry
Issue number5
StatePublished - Jun 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience


  • E2F
  • adult neurogenesis
  • cell cycle
  • synaptic proteins
  • transgenic mice


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