Taurine-activated chloride currents in the rat sacral dorsal commissural neurons

Dian Shi Wang, Tian Le Xu, Zhi Ping Pang, Ji Shuo Li, Norio Akaike

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The electrophysiological and pharmacological properties of taurine (Tau)-activated Cl- currents (I(Tau)) were investigated in the dissociated rat sacral dorsal commissural nucleus (SDCN) neurons using the nystatin perforated patch recording configuration under voltage-clamp conditions. The reversal potential of I(Tau) was close to the Cl- equilibrium potential. The I(Tau) was not affected by a preceding GABA response but cross-desensitized by a preceding glycine (Gly) response. Strychnine (STR), picrotoxin (PIC), bicuculline (BIC) and Zn2+ suppressed the I(Tau) in a concentration- dependent manner. The pharmacology of the I(Tau) and Gly-induced response (I(Gly)) was similar, though Zn2+ inhibition on I(Tau) differed from that on I(Gly) in being much slower in recovery. Serotonin potentiated the I(Tau) via protein kinase C. The results indicate that both Tau and Gly act on a strychnine-sensitive site to open the same Cl- channels in the SDCN neurons, and suggest that Tau may act as a functional neurotransmitter in the mammalian SDCN.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalBrain research
Volume792
Issue number1
DOIs
StatePublished - May 4 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • Acutely dissociated neurons
  • Chloride current
  • Nystatin perforated patch clamp
  • Sacral dorsal commissural nucleus
  • Strychnine-sensitive glycine receptor
  • Taurine

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