TCE degradation in a methanotrophic attached‐film bioreactor

Donna E. Fennell, Yarrow M. Nelson, Sheila E. Underhill, Thomas E. White, William J. Jewell

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Trichloroethene was degraded in expanded‐bed bioreactors operated with mixed‐culture methanotrophic attached films. Biomass concentrations of 8 to 75 g volatile solids (VS) per liter static bed (Lsb) were observed. Batch TCE degradation rates at 35°C followed the Michaelis–Menten model, and a maximum TCE degradation rate (qmax) of 10.6 mg TCE/gVS · day and a half velocity coefficient (KS) of 2.8 mg TCE/L were predicted. Continuous‐flow kinetics also followed the Michaelis–Menten model, but other parameters may be limiting, such as dissolved copper and dissolved methane—qmax and KS were 2.9 mg TCE/gVS · day and 1.5 mg TCE/L, respectively, at low copper concentrations (0.003 to 0.006 mg Cu/L). The maximum rates decreased substantially with small increases in dissolved copper. Methane consumption during continuous‐flow operation varied from 23 to 1200 g CH4/g TCE degraded. Increasing the influent dissolved methane concentration from 0.01 mg/L to 5.4 mg/L reduced the TCE degradation rate by nearly an order of magnitude at 21°C. Exposure of biofilms to 1.4 mg/L tetrachloroethene (PCE) at 35°C resulted in the loss of methane utilization ability. Tests with methanotrophs grown on granular activated carbon indicated that lower effluent TCE concentrations could be obtained. The low efficiencies of TCE removal and low degradation rates obtained at 35°C suggest that additional improvements will be necessary to make methanotrophic TCE treatment attractive. © 1993 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)859-872
Number of pages14
JournalBiotechnology and Bioengineering
Volume42
Issue number7
DOIs
StatePublished - Sep 20 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Keywords

  • attached film
  • biodegradation
  • expanded bed
  • methanotroph
  • trichloroethane

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