Temporal changes in intracellular free calcium levels in the developing neuroepithelium during neurulation in the chick

Joseph Martin, Robert G. Nagele, Lee Hsin-yi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Intracellular free calcium ion (Ca2+) levels of the developing chick neuroepithelium during neural tube closure (Hamburger and Hamilton stages 3-11 of embryonic development) were determined using the Hydrophobie acetoxymethyl ester of the fluorescent dye fura-2 (fura-2/AM). Temporal changes in the free Ca2+ level in neuroepithelial cells are correlated with the degree of folding of the neuroepithelium. The concentration of intracellular Ca2+ in the neuroepithelium reaches its highest level when apposing neural folds are actively making contact.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume107
Issue number4
DOIs
StatePublished - Jan 1 1994

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Neurulation
Fura-2
Neuroepithelial Cells
Calcium
Neural Tube
Neural Crest
Fluorescent Dyes
Embryonic Development
Esters
Ions
2-(2-(2-chloro-3-(2-(3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl)-vinyl)-cyclohex-2-enylidene)-ethylidene)-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid

All Science Journal Classification (ASJC) codes

  • Physiology

Keywords

  • Intracellular free calcium
  • Neurulation

Cite this

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Temporal changes in intracellular free calcium levels in the developing neuroepithelium during neurulation in the chick. / Martin, Joseph; Nagele, Robert G.; Hsin-yi, Lee.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 107, No. 4, 01.01.1994, p. 655-659.

Research output: Contribution to journalArticle

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