Temporal phosphorylation of the Drosophila period protein

Isaac Edery, Laurence J. Zwiebel, Marie E. Dembinska, Michael Rosbash

Research output: Contribution to journalArticlepeer-review

361 Scopus citations


The period gene (per) is required for Drosophila melanogaster to manifest circadian (≃24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.

Original languageEnglish (US)
Pages (from-to)2260-2264
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
StatePublished - Mar 15 1994

All Science Journal Classification (ASJC) codes

  • General


  • circadian rhythms
  • epitope tag
  • per
  • post-transcriptional regulation

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