Temporal phosphorylation of the Drosophila period protein

Isaac Edery; Laurence J. Zwiebel; Marie E. Dembinska; Michael Rosbash

doi:10.1073/pnas.91.6.2260

Temporal phosphorylation of the Drosophila period protein

Isaac Edery, Laurence J. Zwiebel, Marie E. Dembinska, Michael Rosbash

Research output: Contribution to journal › Article › peer-review

361 Scopus citations

Abstract

The period gene (per) is required for Drosophila melanogaster to manifest circadian (≃24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.

Original language	English (US)
Pages (from-to)	2260-2264
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	6
DOIs	https://doi.org/10.1073/pnas.91.6.2260
State	Published - Mar 15 1994

All Science Journal Classification (ASJC) codes

General

Keywords

circadian rhythms
epitope tag
per
post-transcriptional regulation

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10.1073/pnas.91.6.2260

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T1 - Temporal phosphorylation of the Drosophila period protein

AU - Edery, Isaac

AU - Zwiebel, Laurence J.

AU - Dembinska, Marie E.

AU - Rosbash, Michael

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AB - The period gene (per) is required for Drosophila melanogaster to manifest circadian (≃24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.

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KW - post-transcriptional regulation

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