Terminal adenylation in the synthesis of RNA by Q beta replicase.

J. N. Bausch, F. R. Kramer, E. A. Miele, C. Dobkin, D. R. Mills

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We investigated the apparent requirement that Q beta replicase must add a nontemplated adenosine to the 3' end of newly synthesized RNA strands. We used abbreviated MDV-1 (+)-RNA templates that lacked either 62 or 63 nucleotides at their 5' end in Q beta replicase reactions. The MDV-1 (-)-RNA strands synthesized from these abbreviated (+)-strand templates were released from the replication complex, yet they did not possess a nontemplated 3'-terminal adenosine. These results imply that, despite observations that all naturally occurring RNAs synthesized by Q beta replicase possess a nontemplated 3'-adenosine, the addition of an extra adenosine is not an obligate step for the release of completed strands. Since the abbreviated templates lacked a normal 5' end, it is probable that a particular sequence at the 5' end of the template is required for terminal adenylation to occur.

Original languageEnglish (US)
Pages (from-to)1978-1984
Number of pages7
JournalJournal of Biological Chemistry
Issue number3
StatePublished - Feb 10 1983

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Bausch, J. N., Kramer, F. R., Miele, E. A., Dobkin, C., & Mills, D. R. (1983). Terminal adenylation in the synthesis of RNA by Q beta replicase. Journal of Biological Chemistry, 258(3), 1978-1984.